Human IL-18 ELISA Kit | IL18 elisa kit

Human IL-18 PicoKine ELISA Kit

Cat. Num. AAA175863

• Gene Names: IL18; IGIF; IL-18; IL-1g; IL1F4
• Reactivity: Human
• Synonyms: IL-18; Human IL-18 PicoKine ELISA Kit; Interleukin-18; interleukin 18; Iboctadekin; IFN gamma inducing factor; IFN-gamma-inducing factor; IGIF; IL 1 gamma; IL 18; IL 1g; IL-1 gamma; IL1 gamma; IL18; IL18 protein; IL18_HUMAN; IL1F4; IL1g; IL1gamma; ILIF4; Interferon gamma inducing factor; Interferon gamma-inducing factor; Interleukin 1 gamma; Interleukin 18; Interleukin-1 gamma; Interleukin18; MGC12320; IL18 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human IL18
• Sequence Length: 193

• Samples: Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
• Detection Range: 15.6 pg/ml - 1,000 pg/ml
• Sensitivity: <1 pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for IL18 elisa kit

Principle of the Assay: The Picokine™ Human IL18 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human IL18 with a 96-well strip plate that is pre-coated with antibody specific for IL18. The detection antibody is a biotinylated antibody specific for IL18. The capture antibody is monoclonal antibody from mouse and the detection antibody is monoclonal antibody from mouse. The kit contains recombinant Human IL18 with immunogen: Expression system for standard: E.coli; Immunogen sequence: Y33-D197. The kit is analytically validated with ready-to-use reagents. To measure Human IL18, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human IL18 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human IL18 in the sample.

Background/Introduction: Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3

NCBI and Uniprot Product Information

• NCBI GI #: 3219817
• NCBI GeneID: 3606
• UniProt Accession #: Q14116; O75599; Q6FGY3; Q6WWJ7
• Molecular Weight: 21,896 Da
• NCBI Official Full Name: Interleukin-18
• NCBI Official Synonym Full Names: interleukin 18
• NCBI Official Symbol: IL18
• NCBI Official Synonym Symbols: IGIF; IL-18; IL-1g; IL1F4
• NCBI Protein Information: interleukin-18; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)
• UniProt Protein Name: Interleukin-18
• Protein Family: Interleukin
• UniProt Gene Name: IL18
• UniProt Synonym Gene Names: IGIF; IL1F4; IL-18; IFN-gamma-inducing factor; IL-1 gamma
• UniProt Entry Name: IL18_HUMAN

NCBI Description

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

Uniprot Description

IL18: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Belongs to the IL-1 family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 11q22.2-q22.3

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function: protein binding; cytokine activity

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of NK T cell proliferation; natural killer cell activation; positive regulation of NF-kappaB import into nucleus; positive regulation of activated T cell proliferation; chemokine biosynthetic process; cell-cell signaling; lipopolysaccharide-mediated signaling pathway; granulocyte macrophage colony-stimulating factor biosynthetic process; angiogenesis; inflammatory response; positive regulation of interleukin-17 production; regulation of cell adhesion; positive regulation of natural killer cell proliferation; T-helper 1 type immune response; T-helper 2 type immune response; interleukin-2 biosynthetic process; MAPKKK cascade; interleukin-13 biosynthetic process; sleep; positive regulation of tissue remodeling; positive regulation of interferon-gamma production; interferon-gamma biosynthetic process; immune response; negative regulation of myoblast differentiation; positive regulation of inflammatory response