Rabbit anti-Human ARPC1A Polyclonal Antibody | anti-ARPC1A antibody

ARPC1A, ID (Actin-related Protein 2/3 Complex Subunit 1A, SOP2-like Protein, SOP2L) (MaxLight 550)

Cat. Num. AAA6464084

• Gene Names: ARPC1A; Arc40; SOP2L; HEL-68; SOP2Hs; HEL-S-307
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ARPC1A; Polyclonal Antibody; ID (Actin-related Protein 2/3 Complex Subunit 1A; SOP2-like Protein; SOP2L) (MaxLight 550); anti-ARPC1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ARPC1A.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
• Sequence Length: 370

• Applicable Applications for anti-ARPC1A antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human ARPC1A (KLH).
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ARPC1A antibody (Center) in Y79 cell line lysates (35ug/lane). ARPC1A (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of hela cells using ARPC1A Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(ARPC1A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ARPC1A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-ARPC1A antibody

Antibodies; Actin Related Proteins

References

1. Laurila, E., Genes Chromosomes Cancer 48(4), 330-339 (2009). 2. Machesky, L.M., Biochem. J. 328(PT 1), 105-112 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 22907052
• NCBI GeneID: 10552
• NCBI Accession #: NP_006400
• NCBI GenBank Nucleotide #: NP_006400.2
• UniProt Accession #: Q92747
• Molecular Weight: 41.569
• NCBI Official Full Name: actin-related protein 2/3 complex subunit 1A isoform 1
• NCBI Official Synonym Full Names: actin related protein 2/3 complex subunit 1A
• NCBI Official Symbol: ARPC1A
• NCBI Official Synonym Symbols: Arc40; SOP2L; HEL-68; SOP2Hs; HEL-S-307
• NCBI Protein Information: actin-related protein 2/3 complex subunit 1A
• UniProt Protein Name: Actin-related protein 2/3 complex subunit 1A
• Protein Family: Actin-related protein 2/3 complex
• UniProt Gene Name: ARPC1A
• UniProt Synonym Gene Names: SOP2L
• UniProt Entry Name: ARC1A_HUMAN

NCBI Description

This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

ARPC1A: Probably functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Belongs to the WD repeat ARPC1 family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: Arp2/3 protein complex; cytosol; actin cytoskeleton

Molecular Function: actin filament binding; actin binding

Biological Process: axon guidance; ephrin receptor signaling pathway; innate immune response; actin cytoskeleton organization and biogenesis