Rabbit IkB-epsilon Polyclonal Antibody | anti-IkB-epsilon antibody

IkB-epsilon (Ab-22) Antibody

Cat. Num. AAA9408330

• Gene Names: NFKBIE; IKBE
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Synonyms: IkB-epsilon; Polyclonal Antibody; IkB-epsilon (Ab-22) Antibody; IkB-e (Ab-22) Antibody; I-kappa-B-epsilon; anti-IkB-epsilon antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total IkB-e protein.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 500

• Applicable Applications for anti-IkB-epsilon antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around aa. 20~24 (I-E-S-L-R) derived from Human IkB-e.
• Target Name: IkB-e
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from Hela, 293 and HepG2 cells using IkB-e(Ab-22) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-e(Ab-22) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Related Product Information for anti-IkB-epsilon antibody

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.

Product Categories/Family for anti-IkB-epsilon antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 71274109
• NCBI GeneID: 4794
• NCBI Accession #: NP_004547.2
• NCBI GenBank Nucleotide #: NM_004556.2
• UniProt Accession #: O00221; Q5T9V9
• Molecular Weight: 52,864 Da
• NCBI Official Full Name: NF-kappa-B inhibitor epsilon
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon
• NCBI Official Symbol: NFKBIE
• NCBI Official Synonym Symbols: IKBE
• NCBI Protein Information: NF-kappa-B inhibitor epsilon
• UniProt Protein Name: NF-kappa-B inhibitor epsilon
• UniProt Gene Name: NFKBIE
• UniProt Synonym Gene Names: IKBE; NF-kappa-BIE; IkB-E; IkB-epsilon; IkappaBepsilon
• UniProt Entry Name: IKBE_HUMAN

NCBI Description

The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011]

Uniprot Description

IkB-epsilon: Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes. Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit. Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages. Belongs to the NF-kappa-B inhibitor family.

Protein type: DNA-binding; Inhibitor

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; cytoplasm; cytosol

Molecular Function: protein binding

Biological Process: cytoplasmic sequestering of transcription factor; D-serine transport