Rabbit anti-Human, Mouse NFKBIE Polyclonal Antibody | anti-NFKBIE antibody

NFKBIE Antibody

Cat. Num. AAA7045189

• Gene Names: NFKBIE; IKBE
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: NFKBIE; Polyclonal Antibody; NFKBIE Antibody; NF-kappa-B inhibitor epsilon; NF-kappa-BIE; I-kappa-B-epsilon; IkB-E; IkB-epsilon; IkappaBepsilon; IKBE; anti-NFKBIE antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 500

• Applicable Applications for anti-NFKBIE antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Species: Human
• Immunogen: Recombinant human NF-kappa-B inhibitor epsilon protein (140-280AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-7155 / sc-7156
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: NFKBIE antibody at 4.17ug/mlLane 1: Mouse liver tissueLane 2: Hela whole cell lysateLane 3: Jurkat whole cell lysateLane 4: A375 whole cell lysateLane 5: 293T whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 53 kDaObserved band size: 53 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human thyroid tissue MBS7045189 at dilution 1:100)

Related Product Information for anti-NFKBIE antibody

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.

NCBI and Uniprot Product Information

• NCBI GI #: 71274109
• NCBI GeneID: 4794
• NCBI Accession #: NP_004547.2
• NCBI GenBank Nucleotide #: NM_004556.2
• UniProt Accession #: O00221; Q5T9V9
• Molecular Weight: 52,864 Da
• NCBI Official Full Name: NF-kappa-B inhibitor epsilon
• NCBI Official Synonym Full Names: NFKB inhibitor epsilon
• NCBI Official Symbol: NFKBIE
• NCBI Official Synonym Symbols: IKBE
• NCBI Protein Information: NF-kappa-B inhibitor epsilon
• UniProt Protein Name: NF-kappa-B inhibitor epsilon
• Protein Family: NF-kappa-B inhibitor
• UniProt Gene Name: NFKBIE
• UniProt Synonym Gene Names: IKBE; NF-kappa-BIE; IkB-E; IkB-epsilon; IkappaBepsilon
• UniProt Entry Name: IKBE_HUMAN

NCBI Description

The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011]

Uniprot Description

IkB-epsilon: Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes. Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit. Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages. Belongs to the NF-kappa-B inhibitor family.

Protein type: DNA-binding; Inhibitor

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding

Biological Process: cytoplasmic sequestering of transcription factor