IgG Fc fragment Secondary Antibody | IgG secondary antibody

Rabbit anti-Human IgG Fc fragment polyclonal Antibody, HRP conjugated

Cat. Num. AAA719658

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purification Method: Caprylic Acid Ammonium Sulfate Precipitation purified
• Synonyms: IgG Fc fragment; Polyclonal Secondary Antibody; Rabbit anti-Human IgG Fc fragment polyclonal Antibody; HRP conjugated; fragment crystallizable region; IgG secondary antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purification Method: Caprylic Acid Ammonium Sulfate Precipitation purified
• Form/Format: Liquid

• Applicable Applications for IgG secondary antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:500-1:5000
• Immunogen: Human IgG Fc fragment
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: HRP conjugated
• Product Type: Secondary Antibody
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in Recombinantprotein (80ng, 40ng, 20ng, 10ng)All lanes: Human IgG Fc antibody;HRPconjugated at 1:1000Predicted band size: 35 kDaObserved band size: 35 kDa)

Related Product Information for IgG secondary antibody

Relevance: The fragment crystallizable region (Fc region) is the tail region of an antibody that interacts with cell surface receptors called Fc receptors and some proteins of the complement system. This property allows antibodies to activate the immune system. InIgG, IgA and IgD antibody isotypes, the Fc region is composed of two identical protein fragments, derived from the second and third constant domains of the antibody two heavy chains; IgM and IgE Fc regions contain three heavy chain constant domains (CH domains 2-4) in each polypeptide chain. The Fc regions of IgGs bear a highly conserved N-glycosylation site. Glycosylation of the Fc fragment is essential for Fc receptor-mediated activity. The N-glycans attached to this site are predominantly core-fucosylated diantennary structures of the complex type. In addition, small amounts of these N-glycans also bear bisecting GlcNAc and alpha-2,6 linked sialic acid residues.

NCBI Description

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]

Uniprot Description

FCGR2A: low affinity receptor for the Fc region of IgGs. Binding to IgG initiates cellular responses against pathogens and soluble antigens. A type I membrane protein found on monocytes, neutrophils and platelets. SHP-1 associates with this protein to modulate signaling events in myeloid cells.

Protein type: Cell surface; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: plasma membrane; integral to membrane

Molecular Function: IgG binding

Biological Process: innate immune response

Disease: Systemic Lupus Erythematosus; Malaria, Susceptibility To