Western Blot (WB)
(All lanes : Anti-alpha Tubulin Antibody at 1:2000 dilutionLane 1: 293T/17 whole cell lysateLane 2: A431 whole cell lysateLane 3: Hela whole cell lysateLane 4: HepG2 whole cell lysateLane 5: Jurkat whole cell lysateLane 6: Raji whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit alpha Tubulin Polyclonal Antibody | anti-TUBA1B antibody
alpha Tubulin Antibody
Gene Names
TUBA1B; K-ALPHA-1
Reactivity
Human; Predicted Reactivity: Bovine, Chicken, Human, Mouse, Pig, Rat
Applications
Western Blot, ELISA
Synonyms
alpha Tubulin; Polyclonal Antibody; alpha Tubulin Antibody; Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain; TUBA1B; anti-TUBA1B antibody
Host
Rabbit
Reactivity
Human; Predicted Reactivity: Bovine, Chicken, Human, Mouse, Pig, Rat
Clonality
Polyclonal
Isotype
Ig
Specificity
This alpha Tubulin antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 408-439 amino acids from human alpha Tubulin.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
451
Applicable Applications for anti-TUBA1B antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB:~~1:2000
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Cellular Location
Cytoplasm, cytoskeleton.
Antigen Type
Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(All lanes : Anti-alpha Tubulin Antibody at 1:2000 dilutionLane 1: 293T/17 whole cell lysateLane 2: A431 whole cell lysateLane 3: Hela whole cell lysateLane 4: HepG2 whole cell lysateLane 5: Jurkat whole cell lysateLane 6: Raji whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-alpha Tubulin Antibody at 1:2000 dilutionLane 1: 293T/17 whole cell lysateLane 2: A431 whole cell lysateLane 3: Hela whole cell lysateLane 4: HepG2 whole cell lysateLane 5: Jurkat whole cell lysateLane 6: Raji whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-TUBA1B antibody
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Product Categories/Family for anti-TUBA1B antibody
References
Cowan N.J.,et al.Mol. Cell. Biol. 3:1738-1745 (1983). de Hostos E.L.,et al.Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases. Li Q.,et al.Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases. Lubec G.,et al.Submitted (DEC-2008) to UniProtKB. Bienvenut W.V.,et al.Submitted (MAR-2009) to UniProtKB.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50152 Da
NCBI Official Full Name
tubulin alpha-1B chain
NCBI Official Synonym Full Names
tubulin alpha 1b
NCBI Official Symbol
TUBA1B
NCBI Official Synonym Symbols
K-ALPHA-1
NCBI Protein Information
tubulin alpha-1B chain
UniProt Protein Name
Tubulin alpha-1B chain
UniProt Gene Name
TUBA1B
UniProt Entry Name
TBA1B_HUMAN
Uniprot Description
TUBA1B: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 12q13.12
Cellular Component: cytoplasmic microtubule; microtubule; myelin sheath
Molecular Function: double-stranded RNA binding; GTP binding; GTPase activity; protein binding; structural constituent of cytoskeleton; structural molecule activity; ubiquitin protein ligase binding
Biological Process: cell division; cytoskeleton-dependent intracellular transport; metabolic process; microtubule cytoskeleton organization and biogenesis; microtubule-based process
Research Articles on TUBA1B
Similar Products
Product Notes
The TUBA1B tuba1b (Catalog #AAA9217273) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The alpha Tubulin Antibody reacts with Human; Predicted Reactivity: Bovine, Chicken, Human, Mouse, Pig, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's alpha Tubulin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB:~~1:2000. Researchers should empirically determine the suitability of the TUBA1B tuba1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha Tubulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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