Western Blot (WB)
((1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat IGF2BP2 Polyclonal Antibody | anti-IGF2BP2 antibody
Goat anti-IGF2BP2 Antibody
Western Blot: Approx. 60-70kDa band observed in lysates of cell line HepG2 (calculated MW of 66.1kDa according to NP_006539.3). In transfected HEK293 transiently expressing IGF2BP2 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing IGF2BP2 (RC205673) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing IGF2BP2 (RC205673) and probed (mock transfection in first lane), tested by Origene.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternative promoter usage and alternate splicing result in multiple variants encoding different isoforms. [provided by RefSeq, May 2014]
Uniprot Description
IGF2BP2: Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs. Belongs to the RRM IMP/VICKZ family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding; Translation
Chromosomal Location of Human Ortholog: 3q27.2
Cellular Component: cytoplasm; cytoskeleton; cytosol; nucleus
Molecular Function: mRNA 3'-UTR binding; mRNA 5'-UTR binding; protein binding; translation regulator activity
Biological Process: anatomical structure morphogenesis; gene expression; negative regulation of translation; regulation of cytokine biosynthetic process
Disease: Diabetes Mellitus, Noninsulin-dependent
Research Articles on IGF2BP2
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Product Notes
The IGF2BP2 igf2bp2 (Catalog #AAA421577) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-IGF2BP2 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's IGF2BP2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx. 60-70kDa band observed in lysates of cell line HepG2 (calculated MW of 66.1kDa according to NP_006539.3). In transfected HEK293 transiently expressing IGF2BP2 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the IGF2BP2 igf2bp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KQQEQKYPQG VASQR. It is sometimes possible for the material contained within the vial of "IGF2BP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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