Immunohistochemistry (IHC)
(MBS9609279 at 1/100 staining Human cervical cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human IFIT2 Polyclonal Antibody | anti-IFIT2 antibody
IFIT2 Antibody
Gene Names
IFIT2; P54; G10P2; IFI54; ISG54; cig42; IFI-54; IFIT-2; GARG-39; IFI-54K; ISG-54K; ISG-54 K
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
IFIT2; Polyclonal Antibody; IFIT2 Antibody; CIG-42; cig42; G10P2; GARG 39; Glucocorticoid attenuated response gene 39; IFI 54; IFI 54K; IFI-54K; IFI54; IFIT 2; IFIT-2; IFIT2_HUMAN; Interferon induced 54 kDa protein; Interferon induced protein 54; Interferon induced protein with tetratricopeptide repeats 2; Interferon; alpha inducible protein (MW 54kD); Interferon-induced 54 kDa protein; Interferon-induced protein with tetratricopeptide repeats 2; ISG 54 K; ISG 54K; ISG-54 K; ISG54; anti-IFIT2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
IFIT2 antibody detects endogenous levels of total IFIT2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
472
Applicable Applications for anti-IFIT2 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human IFIT2
Subcellular Location
Endoplasmic Reticulum
Tissue Specificity
By type I interferons, dsRNAs and viruses.
Predicted Cross Reactivity
Pig
Similarity
Pig (80%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9609279 at 1/100 staining Human cervical cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9609279 at 1/100 staining Human cervical cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis IFIT2 using HeLa whole cell lysates)
Western Blot (WB)
(Western blot analysis IFIT2 using HeLa whole cell lysates)
Related Product Information for anti-IFIT2 antibody
Function: IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Binds AU-rich viral RNAs, with or without 5' triphosphorylation, RNA-binding is required for antiviral activity. Can promote apoptosis.
Subunit Structure: Domain-swapped homodimer. Component of an interferon-dependent multiprotein complex, at least composed of IFIT1, IFIT2 and IFIT3. Interacts with IFIT1 and IFIT3. Interacts with TMEM173/MITA and disrupts its interaction with MAVS or TBK1. Interacts with EIF3E and EIF3C.
Similarity: The C-terminal part folds into a super-helical structure and has an extensively positively-charged nucleotide-binding channel on its inner surface. Belongs to the IFIT family.
Subunit Structure: Domain-swapped homodimer. Component of an interferon-dependent multiprotein complex, at least composed of IFIT1, IFIT2 and IFIT3. Interacts with IFIT1 and IFIT3. Interacts with TMEM173/MITA and disrupts its interaction with MAVS or TBK1. Interacts with EIF3E and EIF3C.
Similarity: The C-terminal part folds into a super-helical structure and has an extensively positively-charged nucleotide-binding channel on its inner surface. Belongs to the IFIT family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 55 kDa
Predicted: 55 kDa
Predicted: 55 kDa
NCBI Official Full Name
interferon-induced protein with tetratricopeptide repeats 2
NCBI Official Synonym Full Names
interferon induced protein with tetratricopeptide repeats 2
NCBI Official Symbol
IFIT2
NCBI Official Synonym Symbols
P54; G10P2; IFI54; ISG54; cig42; IFI-54; IFIT-2; GARG-39; IFI-54K; ISG-54K; ISG-54 K
NCBI Protein Information
interferon-induced protein with tetratricopeptide repeats 2
UniProt Protein Name
Interferon-induced protein with tetratricopeptide repeats 2
Protein Family
UniProt Gene Name
IFIT2
UniProt Synonym Gene Names
CIG-42; G10P2; IFI54; ISG54; IFIT-2; IFI-54K; P54
Uniprot Description
IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Binds AU-rich viral RNAs, with or without 5' triphosphorylation, RNA-binding is required for antiviral activity. Can promote apoptosis.
Research Articles on IFIT2
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Product Notes
The IFIT2 ifit2 (Catalog #AAA9609279) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IFIT2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IFIT2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the IFIT2 ifit2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFIT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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