Rabbit anti-Human IFI30 Polyclonal Antibody | anti-IFI30 antibody

Anti-IFI30 Antibody, Rabbit Polyclonal

Cat. Num. AAA8106560

• Gene Names: IFI30; GILT; IP30; IP-30; IFI-30
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: IFI30; Polyclonal Antibody; Anti-IFI30 Antibody; Rabbit Polyclonal; IFI30 Antibody; Rabbit PAb; Antigen Affinity Purified; interferon gamma inducible protein 30; Anti-GILT Antibody; Anti-IFI-30 Antibody; Anti-IP-30 Antibody; Anti-IP30 Antibody; anti-IFI30 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human IFI30
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-IFI30 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000
• Immunogen: Recombinant Human IFI30 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human IFI30 (rh IFI30). IFI30 specific IgG was purified by Human IFI30 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-IFI30 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:29 kDaObserved band size:30 kDa)

Related Product Information for anti-IFI30 antibody

IFI30 belongs to the GILT family. This family includes the two characterized human gamma-interferon-inducible lysosomal thiol reductase (GILT) sequences: P13284 and Q9UL08. It also contains several other eukaryotic putative proteins with similarity to GILT. The aligned region contains three conserved cysteine residues. Besides, the two GILT sequences possess a C-X(2)-C motif that is shared by some of the other sequences in the family. This motif is thought to be associated with disulfide bond reduction. IFI30 is a lysosomal thiol reductase that can reduce protein disulfide bonds. It facilitates the generation of MHC class II-restricted epitopes from disulfide bond-containing antigens by the endocytic reduction of disulfide bonds. It also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or cross-presentation. IFI30 may facilitate the complete unfolding of proteins destined for lysosomal degradation and plays an important role in antigen processing.

References

Haque MA, et al. (2002) Absence of gamma-Interferon-inducible Lysosomal Thiol Reductase in Melanomas Disrupts T Cell Recognition of Select Immunodominant Epitopes. J Exp Med. 195(10):1267-77.Phan UT, et al. (2000) Gamma-interferon-inducible lysosomal thiol reductase (GILT). Maturation, activity, and mechanism of action. J Biol Chem. 275(34):25907-14.Phan UT, et al. (2001) Multiple species express thiol oxidoreductases related to GILT. Immunogenetics. 53(4):342-6.

NCBI and Uniprot Product Information

• NCBI GI #: 29150261
• NCBI GeneID: 10437
• NCBI Accession #: NP_006323.2
• NCBI GenBank Nucleotide #: NM_006332.4
• UniProt Accession #: P13284; Q76MF9; Q8NEI4; Q8WU77; Q9UL08
• Molecular Weight: 27,964 Da
• NCBI Official Full Name: gamma-interferon-inducible lysosomal thiol reductase preproprotein
• NCBI Official Synonym Full Names: interferon, gamma-inducible protein 30
• NCBI Official Symbol: IFI30
• NCBI Official Synonym Symbols: GILT; IP30; IP-30; IFI-30
• NCBI Protein Information: gamma-interferon-inducible lysosomal thiol reductase; gamma-interferon-inducible protein IP-30; interferon gamma-inducible protein 30 preproprotein; legumaturain
• UniProt Protein Name: Gamma-interferon-inducible lysosomal thiol reductase
• Protein Family: Gamma-interferon-inducible lysosomal thiol reductase
• UniProt Gene Name: IFI30
• UniProt Synonym Gene Names: GILT; IP30
• UniProt Entry Name: GILT_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. [provided by RefSeq, Jul 2008]

Uniprot Description

IFI30: Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II- restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation. Belongs to the GILT family.

Protein type: Secreted, signal peptide; EC 1.8.-.-; Secreted

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: lysosomal lumen; intracellular membrane-bound organelle; lysosome; cytoplasm; extracellular region; plasma membrane; cell junction

Molecular Function: oxidoreductase activity, acting on sulfur group of donors

Biological Process: protein stabilization; negative regulation of fibroblast proliferation; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class II