Testing Data #1
(Fig.l: FC analysis of BOSC23 cells using MUS Cat # MBS468031. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAMS (red curve) or an irrelevant protein (control transfectant). Binding of MUS was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CEACAMS transfected cells.)
Mouse anti-Human CEACAM5,6 Monoclonal Antibody | anti-CEACAM5 antibody
anti-human CEACAM5,6 monoclonal antibody
ELISA: 1:200 - 1:400
Western Blot: 4ug/ml
Flow Cytometry: 1.2 ug/106 cells
CELISA: 1:200
For each application a titration should be performed to determine the optimal concentration.
Testing Data #1
(Fig.l: FC analysis of BOSC23 cells using MUS Cat # MBS468031. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAMS (red curve) or an irrelevant protein (control transfectant). Binding of MUS was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CEACAMS transfected cells.)
Testing Data #1
(Fig.l: FC analysis of BOSC23 cells using MUS Cat # MBS468031. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAMS (red curve) or an irrelevant protein (control transfectant). Binding of MUS was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CEACAMS transfected cells.)
Testing Data #2
(Fig.2: FACS analysis of BOSC23 cells using MUS Cat.# MBS468031. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM6 (red curve) or an irrelevant protein (control transfectant). Binding of MUS was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM6 transfected cells.)
Testing Data #2
(Fig.2: FACS analysis of BOSC23 cells using MUS Cat.# MBS468031. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM6 (red curve) or an irrelevant protein (control transfectant). Binding of MUS was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM6 transfected cells.)
Testing Data #3
(Fig. 3: Specificity testing of MUS. BOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, 3, 5, 6, 7, 8 or a recombinant transmembrane-anchored PSG1 fusion protein. Recognition of CEACAM4 was tested on CHO cells stably transfected with a CEACAM4 expression vector. Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: 80H3; PSG: BAP1; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified MUS was tested on all CEACAM transfectants. A positive signal was obtained with CEACAM5 and CEACAM6 expressing cells (red curves).)
Testing Data #3
(Fig. 3: Specificity testing of MUS. BOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, 3, 5, 6, 7, 8 or a recombinant transmembrane-anchored PSG1 fusion protein. Recognition of CEACAM4 was tested on CHO cells stably transfected with a CEACAM4 expression vector. Expression of the constructs was confirmed with monoclonal antibodies known to recognise the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: CAC2; CEACAM8: 80H3; PSG: BAP1; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G purified MUS was tested on all CEACAM transfectants. A positive signal was obtained with CEACAM5 and CEACAM6 expressing cells (red curves).)
Hammarström S (1999). The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. Semin Cancer Biol. 9, 67-81.
Schölzel S, Zimmermann W, Schwarzkopf G, Grunert F, Rogaczewski B, Thompson J (2000). Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas. Am J Pathol 156, 595-605.
Grunert F, AbuHarfeil N, Schwarz K and von Kleist S (1985). Two CEA and three NCA species, although distinguishable by monoclonal antibodies, have nearly identical peptide patterns. Int J Cancer 36, 357-362.
NCBI and Uniprot Product Information
NCBI Description
Members of the CEACAM subfamily, including CEACAM5, belong to the CEA gene family. For general information on the CEA gene family, see CEACAM1 (MIM 109770).[supplied by OMIM, Oct 2009]
Uniprot Description
CEACAM5: Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Belongs to the immunoglobulin superfamily. CEA family.
Protein type: Membrane protein, GPI anchor; Immunoglobulin superfamily; Membrane protein, integral
Chromosomal Location of Human Ortholog: 19q13.1-q13.2
Cellular Component: integral to plasma membrane; basolateral plasma membrane
Molecular Function: identical protein binding; protein homodimerization activity
Biological Process: homotypic cell-cell adhesion; negative regulation of apoptosis
Research Articles on CEACAM5
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Product Notes
The CEACAM5 ceacam5 (Catalog #AAA468031) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CEACAM5,6 monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CEACAM5,6 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), CELISA, ELISA (EIA), Western Blot (WB), Immunohistology (on cryosections). Immunohistology: 1-2 ug/106 cells (on cryosections) ELISA: 1:200 - 1:400 Western Blot: 4ug/ml Flow Cytometry: 1.2 ug/106 cells CELISA: 1:200 For each application a titration should be performed to determine the optimal concentration. Researchers should empirically determine the suitability of the CEACAM5 ceacam5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEACAM5,6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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