Goat IDS Polyclonal Antibody | anti-IDS antibody

Goat Polyclonal to Human IDS

Cat. Num. AAA243534

• Gene Names: IDS; MPS2; SIDS
• Reactivity: Gibbon, Gorilla, Human, Monkey
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: IDS; Polyclonal Antibody; Goat Polyclonal to Human IDS; Anti-IDS Antibody (Internal) IHC-plus; Idursulfase; SIDS; Iduronate 2-sulfatase; MPS2; Human IDS; anti-IDS antibody

• Host: Goat
• Reactivity: Gibbon, Gorilla, Human, Monkey
• Clonality: Polyclonal
• Specificity: Human IDS. This antibody is expected to recognize isoform a (NP_000193.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 550

• Applicable Applications for anti-IDS antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:16000), IHC-P (3.75 ug/ml), WB (0.1 - 0.3 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx. 60kDa band observed in Human Liver lysates (calculated MW of 61.9kDa according to NP_000193.1). Recommended concentration: 0.1-0.3 ug/ml.
• Target Species: Human
• Immunogen Description: Synthetic peptide C-KHFRFRDLEEDP from an internal region of human IDS (NP_000193.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Panda (83%).
• Immunogen Type: Synthetic peptide
• Immunogen: IDS antibody was raised against synthetic peptide C-KHFRFRDLEEDP from an internal region of human IDS (NP_000193.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Panda (83%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-IDS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.)

Western Blot (WB)

(IDS antibody (0.1 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-IDS antibody

Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked Mucopolysaccharidosis Type II, also known as Hunter Syndrome. Iduronate-2-sulfatase has a strong sequence homology with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase. A splice variant of this gene has been described.

NCBI and Uniprot Product Information

• NCBI GI #: 4557659
• NCBI GeneID: 3423
• NCBI Accession #: NP_000193.1
• NCBI GenBank Nucleotide #: NM_000202.6
• UniProt Accession #: P22304; Q14604; Q9BRM3; D3DWT4
• Molecular Weight: 34,893 Da
• NCBI Official Full Name: iduronate 2-sulfatase isoform a preproprotein
• NCBI Official Synonym Full Names: iduronate 2-sulfatase
• NCBI Official Symbol: IDS
• NCBI Official Synonym Symbols: MPS2; SIDS
• NCBI Protein Information: iduronate 2-sulfatase; idursulfase; iduronate 2-sulfatase 14 kDa chain; iduronate 2-sulfatase 42 kDa chain; alpha-L-iduronate sulfate sulfatase
• UniProt Protein Name: Iduronate 2-sulfatase
• Protein Family: Iduronate 2-sulfatase
• UniProt Gene Name: IDS
• UniProt Synonym Gene Names: SIDS; Idursulfase
• UniProt Entry Name: IDS_HUMAN

NCBI Description

The protein encoded by this gene belongs to the sulfatase family, is localized to the lysosome, and is involved in lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease, mucopolysaccharidosis type II, also known as Hunter syndrome. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Aug 2013]

Uniprot Description

IDS: Required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Defects in IDS are the cause of mucopolysaccharidosis type 2 (MPS2); also known as Hunter syndrome. MPS2 is an X-linked lysosomal storage disease characterized by intracellular accumulation of heparan sulfate and dermatan sulfate and their excretion in urine. Most children with MPS2 have a severe form with early somatic abnormalities including skeletal deformities, hepatosplenomegaly, and progressive cardiopulmonary deterioration. A prominent feature is neurological damage that presents as developmental delay and hyperactivity but progresses to mental retardation and dementia. They die before 15 years of age, usually as a result of obstructive airway disease or cardiac failure. In contrast, those with a mild form of MPS2 may survive into adulthood, with attenuated somatic complications and often without mental retardation. Belongs to the sulfatase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Glycan Metabolism - glycosaminoglycan degradation; EC 3.1.6.13; Hydrolase

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: lysosomal lumen

Molecular Function: iduronate-2-sulfatase activity; metal ion binding

Biological Process: chondroitin sulfate metabolic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; chondroitin sulfate catabolic process; carbohydrate metabolic process; pathogenesis

Disease: Mucopolysaccharidosis, Type Ii