Human IDS PicoKine ELISA Kit | IDS elisa kit

Human IDS PicoKine ELISA Kit

Cat. Num. AAA177589

• Gene Names: IDS; MPS2; SIDS
• Reactivity: Human
• Synonyms: IDS PicoKine; Human IDS PicoKine ELISA Kit; Iduronate 2-sulfatase; Ids; Iduronate 2 sulfatase; Iduronate sulfatase; Idursulfase; MPS2; SIDS; iduronate 2-sulfatase; IDS elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human IDS
• Sequence Length: 550

• Assay Range: 156pg/ml-10000pg/ml
• Sensitivity: <15pg/ml
• Sample: For quantitative detection of human IDS in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: NSO, S26-P550
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human IDS standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IDS standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|1581|2903|6320|2052|3475|7895; Standard deviation|102.77|139.34|328.64|155.95|229.35|505.28; CV(%)|6.5|4.8|5.2|7.6|6.6|6.4
• Sample Data: Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000; O.D.|0.028|0.069|0.125|0.215|0.381|0.729|1.433|2.243
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human IDS PicoKine ELISA Kit standard curve )

Related Product Information for IDS elisa kit

Principle of the assay: Human IDS ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IDS has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: S26-P550) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IDS is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IDS amount of sample captured in plate.

Background: Iduronate 2-sulfatase (IDS) is a sulfatase enzyme associated with Hunter syndrome. It encodes a member of the sulfatase family of proteins. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

NCBI and Uniprot Product Information

• NCBI GI #: 4557659
• NCBI GeneID: 3423
• NCBI Accession #: NP_000193.1
• NCBI GenBank Nucleotide #: NM_000202.7
• UniProt Accession #: P22304; Q14604; Q9BRM3; D3DWT4
• Molecular Weight: 34,893 Da
• NCBI Official Full Name: iduronate 2-sulfatase isoform a preproprotein
• NCBI Official Synonym Full Names: iduronate 2-sulfatase
• NCBI Official Symbol: IDS
• NCBI Official Synonym Symbols: MPS2; SIDS
• NCBI Protein Information: iduronate 2-sulfatase
• UniProt Protein Name: Iduronate 2-sulfatase
• Protein Family: Iduronate 2-sulfatase
• UniProt Gene Name: IDS
• UniProt Synonym Gene Names: SIDS; Idursulfase
• UniProt Entry Name: IDS_HUMAN

NCBI Description

This gene encodes a member of the sulfatase family of proteins. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. This enzyme is involved in the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

IDS: Required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Defects in IDS are the cause of mucopolysaccharidosis type 2 (MPS2); also known as Hunter syndrome. MPS2 is an X-linked lysosomal storage disease characterized by intracellular accumulation of heparan sulfate and dermatan sulfate and their excretion in urine. Most children with MPS2 have a severe form with early somatic abnormalities including skeletal deformities, hepatosplenomegaly, and progressive cardiopulmonary deterioration. A prominent feature is neurological damage that presents as developmental delay and hyperactivity but progresses to mental retardation and dementia. They die before 15 years of age, usually as a result of obstructive airway disease or cardiac failure. In contrast, those with a mild form of MPS2 may survive into adulthood, with attenuated somatic complications and often without mental retardation. Belongs to the sulfatase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.6.13; Hydrolase; Glycan Metabolism - glycosaminoglycan degradation

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: lysosomal lumen

Molecular Function: iduronate-2-sulfatase activity; metal ion binding

Biological Process: chondroitin sulfate catabolic process; glycosaminoglycan catabolic process

Disease: Mucopolysaccharidosis, Type Ii