Flow Cytometry (FC/FACS)
(Staining of BALB/c thymocytes with CD4 antibody (eFluor 450), CD8a antibody and 0.25 ug of Mouse IgG2a K Isotype Control (PE) (left) or 0.25 ug of CD199 antibody (PE) (right). Samples were gated on CD4 single positive (orange histogram) or CD4+CD8+ double positive cells (purple histogram).)
Mouse CD199 Monoclonal Antibody | anti-CD199 antibody
CD199 antibody (PE)
Applications
Flow Cytometry, Functional Assay
Synonyms
CD199; Monoclonal Antibody; CD199 antibody (PE); Monoclonal CD199; Anti-CD199; GPR28; CD-199; CD 199; C-C chemokine receptor type 9; Cmkbr10; CCR-9; anti-CD199 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
CW-1.2
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
200 ug/mL (varies by lot)
Applicable Applications for anti-CD199 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: 5 ul (0.5 ug)/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Biological Significance
The CW-1.2 monoclonal antibody reacts with mouse CCR9, which is the receptor for thymus-expressed chemokine. CCR9 is a member of the G protein coupled receptor supergene family, and is involved in trafficking of T cell progenitors within the thymus. CCR9 expression during thymocyte development commences at the double-negative 3 stage, peaks in the double-positive stage, and is down-regulated in commited CD4+ or CD8+ single-positive thymoyctes. CCR9-deficient mice show a mild impairment in thymoycte development. In the periphery, CCR9 is thought to be expressed on na
Tag/Conjugate
PE
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of BALB/c thymocytes with CD4 antibody (eFluor 450), CD8a antibody and 0.25 ug of Mouse IgG2a K Isotype Control (PE) (left) or 0.25 ug of CD199 antibody (PE) (right). Samples were gated on CD4 single positive (orange histogram) or CD4+CD8+ double positive cells (purple histogram).)
Flow Cytometry (FC/FACS)
(Staining of BALB/c thymocytes with CD4 antibody (eFluor 450), CD8a antibody and 0.25 ug of Mouse IgG2a K Isotype Control (PE) (left) or 0.25 ug of CD199 antibody (PE) (right). Samples were gated on CD4 single positive (orange histogram) or CD4+CD8+ double positive cells (purple histogram).)
Related Product Information for anti-CD199 antibody
Mouse monoclonal CD199 antibody (PE)
Product Categories/Family for anti-CD199 antibody
Similar Products
Product Notes
The CD199 (Catalog #AAA832949) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD199 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: 5 ul (0.5 ug)/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Researchers should empirically determine the suitability of the CD199 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD199, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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