Bovine Insulin-like growth factor 1 receptor ELISA Kit | IGF1R elisa kit

Bovine Insulin-like growth factor 1 receptor ELISA Kit

Cat. Num. AAA289379

• Gene Names: IGF1R; IGFR1
• Reactivity: Bovine
• Synonyms: Insulin-like growth factor 1 receptor; Bovine Insulin-like growth factor 1 receptor ELISA Kit; Insulin-like growth factor I receptor; IGF-I receptor; IGF1R; 2.7.10.1; CD221; IGF1R elisa kit

• Reactivity: Bovine
• Specificity: This assay recognizes recombinant and natural bovineInsulin-like growth factor 1 receptor. No significant cross-reactivity or interference was observed.
• Sequence Length: 1367

• Samples: Serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
• Detection Range: 78.0pg/mL -5000pg/mL.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGF1R elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination ofbovineInsulin-like growth factor 1 receptorconcentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin-like growth factor 1 receptor. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forInsulin-like growth factor 1 receptor and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Insulin-like growth factor 1 receptor, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Insulin-like growth factor 1 receptor in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 347446671
• NCBI GeneID: 281848
• NCBI Accession #: NP_001231541.1
• NCBI GenBank Nucleotide #: NM_001244612.1
• UniProt Accession #: Q05688
• Molecular Weight: 72,511 Da
• NCBI Official Full Name: insulin-like growth factor 1 receptor
• NCBI Official Symbol: IGF1R
• NCBI Official Synonym Symbols: IGFR1
• NCBI Protein Information: insulin-like growth factor 1 receptor
• UniProt Protein Name: Insulin-like growth factor 1 receptor
• Protein Family: Insulin-like growth factor 1 receptor
• UniProt Gene Name: IGF1R
• UniProt Synonym Gene Names: IGF-I receptor
• UniProt Entry Name: IGF1R_BOVIN

Uniprot Description

Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R (). When present in a hybrid receptor with INSR, binds IGF1 ().