Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-2: Human kidney tissue£¬SKOV3 cell Primary antibody: 1/250 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds)
Rabbit anti-Human IDO2 Polyclonal Antibody | anti-IDO2 antibody
IDO2 Antibody
Gene Names
IDO2; INDOL1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
IDO2; Polyclonal Antibody; IDO2 Antibody; INDOL1; anti-IDO2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total IDO2 protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
420
Applicable Applications for anti-IDO2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:200-1:1000
Immunohistochemistry: 1:25-1:100
Immunohistochemistry: 1:25-1:100
Immunogen
Full length fusion protein of human IDO2
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-2: Human kidney tissue£¬SKOV3 cell Primary antibody: 1/250 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds)
Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-2: Human kidney tissue£¬SKOV3 cell Primary antibody: 1/250 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds)
Related Product Information for anti-IDO2 antibody
INDOL1 is also known as IDO2 (indoleamine 2,3-dioxygenase 2) and is a 407 amino acid protein that is expressed in various tissues, including liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, colon and dendritic cells.INDOL1 is selectively inhibited by D-1MT (1-methyl-d-tryptophan), which also inhibits IDO (indoleamine 2,3-dioxygenase) and is significant because IDO expression causes suppression of T cell responses to tumors in dendritic cells. The inhibition of INDOL1 by D-1MT suggests a common function in immunomodulation. In the human INDOL1 gene, two single nucleotide polymorphisms have been detected which abolish the enzymatic function of INDOL1.
Product Categories/Family for anti-IDO2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,610 Da
NCBI Official Full Name
indoleamine 2,3-dioxygenase 2
NCBI Official Synonym Full Names
indoleamine 2,3-dioxygenase 2
NCBI Official Symbol
IDO2
NCBI Official Synonym Symbols
INDOL1
NCBI Protein Information
indoleamine 2,3-dioxygenase 2
UniProt Protein Name
Indoleamine 2,3-dioxygenase 2
Protein Family
UniProt Gene Name
IDO2
UniProt Synonym Gene Names
INDOL1; IDO-2
UniProt Entry Name
I23O2_HUMAN
Uniprot Description
INDOL1: Catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism. Belongs to the indoleamine 2,3-dioxygenase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - tryptophan; EC 1.13.11.-; Oxidoreductase
Chromosomal Location of Human Ortholog: 8p11.21
Cellular Component: cytoplasm; cytosol
Molecular Function: indoleamine 2,3-dioxygenase activity; tryptophan 2,3-dioxygenase activity
Biological Process: tryptophan catabolic process; tryptophan catabolic process to kynurenine
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Product Notes
The IDO2 ido2 (Catalog #AAA9415585) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IDO2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IDO2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:200-1:1000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the IDO2 ido2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IDO2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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