Rabbit anti-Human GLYCTK Polyclonal Antibody | anti-GLYCTK antibody

GLYCTK, NT (GLYCTK, HBEBP4, Glycerate kinase, HBeAg-binding protein 4) (PE)

Cat. Num. AAA6302764

• Gene Names: GLYCTK; HBEBP2; HBEBP4; HBeAgBP4A
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GLYCTK; Polyclonal Antibody; NT (GLYCTK; HBEBP4; Glycerate kinase; HBeAg-binding protein 4) (PE); HBeAg-binding protein 4; anti-GLYCTK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-GLYCTK antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GLYCTK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the N-terminal region of human GLYCTK.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GLYCTK Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the GLYCTK antibody detected the GLYCTK protein (arrow).)

Related Product Information for anti-GLYCTK antibody

This locus encodes a member of the glycerate kinase type-2 family. The encoded enzyme catalyzes the phosphorylation of (R)-glycerate and may be involved in serine degradation and fructose metabolism. Decreased activity of the encoded enzyme may be associated with the disease D-glyceric aciduria. Alternatively spliced transcript variants have been described. [provided by RefSeq].

Product Categories/Family for anti-GLYCTK antibody

Antibodies; Protein Kinases

References

Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009). Guo, J.H., et al. DNA Seq. 17(1):1-7(2006). Fontaine, M., et al. Clin. Chem. 35(10):2148-2151(1989) Van Schaftingen, E. FEBS Lett. 243(2):127-131(1989).

NCBI and Uniprot Product Information

• NCBI GI #: 222418771
• NCBI GeneID: 132158
• NCBI Accession #: NP_001138423.1; NP_660305.2
• NCBI GenBank Nucleotide #: NM_001144951.1
• UniProt Accession #: Q8IVS8; Q0P630; Q2EZ43; Q6Y2K6; Q7Z6G5; Q86YR8; Q8TED2; Q8WTY2
• Molecular Weight: 55,253 Da
• NCBI Official Full Name: glycerate kinase isoform 2
• NCBI Official Synonym Full Names: glycerate kinase
• NCBI Official Symbol: GLYCTK
• NCBI Official Synonym Symbols: HBEBP2; HBEBP4; HBeAgBP4A
• NCBI Protein Information: glycerate kinase; HBeAg binding protein 4; HBeAg-binding protein 2
• UniProt Protein Name: Glycerate kinase
• UniProt Gene Name: GLYCTK
• UniProt Synonym Gene Names: HBEBP4
• UniProt Entry Name: GLCTK_HUMAN

NCBI Description

This locus encodes a member of the glycerate kinase type-2 family. The encoded enzyme catalyzes the phosphorylation of (R)-glycerate and may be involved in serine degradation and fructose metabolism. Decreased activity of the encoded enzyme may be associated with the disease D-glyceric aciduria. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]

Uniprot Description

GLYCTK: Defects in GLYCTK are the cause of D-glyceric aciduria (D-GA). D-GA is a rare metabolic disease characterized by chronic metabolic acidosis and a highly variable clinical phenotype. Clinical features range from an encephalopathic presentation with seizures, microcephaly, severe mental retardation and early death, to milder manifestations with only speech delay or even normal development. Belongs to the glycerate kinase type-2 family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerolipid; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 2.7.1.31; Kinase, other; Amino Acid Metabolism - glycine, serine and threonine

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: mitochondrion; cytoplasm

Molecular Function: protein binding; glycerate kinase activity; ATP binding

Biological Process: protein amino acid phosphorylation

Disease: D-glyceric Aciduria