Mouse anti-Human COL2A1 Monoclonal Antibody | anti-COL2A1 antibody

COL2A1 (Collagen alpha-1(II) Chain, Alpha-1 Type II Collagen, Chondrocalcin) (Biotin)

Cat. Num. AAA6141291

• Gene Names: COL2A1; AOM; ANFH; SEDC; STL1; COL11A3
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: COL2A1; Monoclonal Antibody; COL2A1 (Collagen alpha-1(II) Chain; Alpha-1 Type II Collagen; Chondrocalcin) (Biotin); anti-COL2A1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3H1-F9
• Specificity: Recognizes human COL2A1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-COL2A1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-268 from human COL2A1 (AAH07252) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MSAFAGLGPREKGPDPLQYMRADQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPARTCRDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNPANVPKKNWWSSKSKEKKHIWFGETINGGFHFSYGDDNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIAYLDEAAGNLKKALLIQGSNDVEIRAEGNSRFTYTALKDGCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPMDIGGPEQEFGVDIGPVCFL
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (55.22kD).)

Related Product Information for anti-COL2A1 antibody

Alpha-1 chain of type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutant forms of this protein are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

Product Categories/Family for anti-COL2A1 antibody

Antibodies; Collagen

References

1. The effect of platelet lysate supplementation of a dextran-based hydrogel on cartilage formation. Moreira Teixeira LS, Leijten JC, Wennink JW, Chatterjea AG, Feijen J, van Blitterswijk CA, Dijkstra PJ, Karperien M.Biomaterials. 2012 May;33(14):3651-61. Epub 2012 Feb 18. 2. Raman Microspectroscopy - A Non-Invasive Analysis Tool For Monitoring Of Collagen-Containing Extracellular Matrix Formation in a Medium-Throughput Culture System. Kunstar A, Otto C, Karperien M, van Blitterswijk C, van Apeldoorn A.Tissue Eng Part C Methods. 2011 Mar 16. 3. Enzymatically Crosslinked Dextran-Tyramine Hydrogels as Injectable Scaffolds for Cartilage Tissue Engineering. Jin R, Moreira Teixeira LS, Dijkstra PJ, Zhong Z, van Blitterswijk CA, Karperien M, Feijen J.Tissue Eng Part A. 2010 May 22. 4. EXTRACELLULAR AND MEMBRANE-ASSOCIATED PROSTATE CANCER MARKERS Klee, George G. ;Vasmatzis, George;Kosari, Farhad;Klee, Eric W.FreshPatents.com 5. Synthesis and characterization of hyaluronic acid-poly(ethylene glycol) hydrogels via Michael addition: An injectable biomaterial for cartilage repair. Jin R, Moreira Teixeira LS, Krouwels A, Dijkstra PJ, van Blitterswijk CA, Karperien M, Feijen J.Acta Biomater. 2009 Dec 16.

NCBI and Uniprot Product Information

• NCBI GI #: 13938252
• NCBI GeneID: 1280
• Molecular Weight: 29,781
• NCBI Official Full Name: Homo sapiens collagen, type II, alpha 1, mRNA
• NCBI Official Synonym Full Names: collagen type II alpha 1 chain
• NCBI Official Symbol: COL2A1
• NCBI Official Synonym Symbols: AOM; ANFH; SEDC; STL1; COL11A3
• NCBI Protein Information: collagen alpha-1(II) chain

NCBI Description

This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]