Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lymph with ICOS Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit anti-Human ICOS Polyclonal Antibody | anti-ICOS antibody
ICOS Antibody (C-term)
WB~~1:1000
FC~~1:10~50
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lymph with ICOS Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lymph with ICOS Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB)
(ICOS-S171 western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ICOS antibody detected the ICOS protein (arrow).)
Western Blot (WB)
(ICOS-S171 western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ICOS antibody detected the ICOS protein (arrow).)
Flow Cytometry (FC/FACS)
(ICOS Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ICOS Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Okamoto, T., et al., J. Rheumatol. 30(6):1157-1163 (2003).
Riley, J.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(18):11790-11795 (2002).
Haimila, K.E., et al., Immunogenetics 53(12):1028-1032 (2002).
Witsch, E.J., et al., Eur. J. Immunol. 32(9):2680-2686 (2002).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. [provided by RefSeq, Jul 2008]
Uniprot Description
ICOS: Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up- regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up- regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre- activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. Defects in ICOS are the cause of immunodeficiency common variable type 1 (CVID1). CVID1 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be lo. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 2q33
Cellular Component: integral to plasma membrane; plasma membrane; extracellular region; external side of plasma membrane
Biological Process: cell-cell adhesion; T cell tolerance induction; T cell costimulation; immune response
Disease: Immunodeficiency, Common Variable, 2; Immunodeficiency, Common Variable, 1
Research Articles on ICOS
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Product Notes
The ICOS icos (Catalog #AAA9203882) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 156-185. The ICOS Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ICOS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry-Paraffin (IHC-P). IHC-P~~1:50~100 WB~~1:1000 FC~~1:10~50. Researchers should empirically determine the suitability of the ICOS icos for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ICOS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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