Fibulin 5 (FBLN5) Recombinant Protein | FBLN5 recombinant protein

Recombinant Fibulin 5 (FBLN5)

Cat. Num. AAA2018281

• Gene Names: Fbln5; A55; EVEC; DANCE
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Fibulin 5 (FBLN5); Recombinant Fibulin 5 (FBLN5); FBLN5 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF- D ECRYGYCQQL CANVPGSYSC TCNPGFTLND DGRSCQDVNE CETENPCVQT CVNTYGSFIC RCDPGYELEE DGI
• Sequence Length: 448

• Applicable Applications for FBLN5 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Asp170~Ile243 (Accession # Q9WVH9) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6753824
• NCBI GeneID: 23876
• NCBI Accession #: NP_035942.1
• NCBI GenBank Nucleotide #: NM_011812.4
• UniProt Accession #: Q9WVH9; Q541Z7
• Molecular Weight: 9.7kDa
• NCBI Official Full Name: fibulin-5
• NCBI Official Synonym Full Names: fibulin 5
• NCBI Official Symbol: Fbln5
• NCBI Official Synonym Symbols: A55; EVEC; DANCE
• NCBI Protein Information: fibulin-5; FIBL-5; developmental arteries and neural crest EGF-like protein
• UniProt Protein Name: Fibulin-5
• Protein Family: Fibulin
• UniProt Gene Name: Fbln5
• UniProt Synonym Gene Names: Dance; FIBL-5; Dance
• UniProt Entry Name: FBLN5_MOUSE

Uniprot Description

FBLN5: Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Could be a vascular ligand for integrin receptors and may play a role in vascular development and remodeling. Defects in FBLN5 are the cause of cutis laxa, autosomal dominant, type 2 (ADCL2). A connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. Face, hands, feet, joints, and torso may be differentially affected. Additional variable clinical features are gastrointestinal diverticula, hernia, and genital prolapse. Rare manifestations are pulmonary artery stenosis, aortic aneurysm, bronchiectasis, and emphysema. Defects in FBLN5 are a cause of cutis laxa, autosomal recessive, type 1A (ARCL1A). A connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. Face, hands, feet, joints, and torso may be differentially affected. The clinical spectrum of autosomal recessive cutis laxa is highly heterogeneous with respect to organ involvement and severity. Type I autosomal recessive cutis laxa is a specific, life-threatening disorder with organ involvement, lung atelectasis and emphysema, diverticula of the gastrointestinal and genitourinary systems, and vascular anomalies. Associated cranial anomalies, late closure of the fontanel, joint laxity, hip dislocation, and inguinal hernia have been observed but are uncommon. Defects in FBLN5 are the cause of age-related macular degeneration type 3 (ARMD3). ARMD is a multifactorial disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid (known as drusen) that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Belongs to the fibulin family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: protein C-terminus binding; integrin binding; protein homodimerization activity; calcium ion binding

Biological Process: elastic fiber assembly; secretion; extracellular matrix organization and biogenesis; cell adhesion