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Western Blot (WB) (Western blot analysis of MRPS9 expression in U251 (A), A549 (B) whole cell lysates.)

Rabbit anti-Human MRPS9 Polyclonal Antibody | anti-MRPS9 antibody

Anti-MRPS9 Antibody

Gene Names
MRPS9; S9mt; RPMS9; MRP-S9
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by affinity chromatography.
Synonyms
MRPS9; Polyclonal Antibody; Anti-MRPS9 Antibody; RPMS9; 28S ribosomal protein S9; mitochondrial; MRP-S9; S9mt; anti-MRPS9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of MRPS9 protein.
Purity/Purification
The antibody was purified by affinity chromatography.
Form/Format
Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% Sodium Azide.
Sequence Length
396
Applicable Applications for anti-MRPS9 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MRPS9. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of MRPS9 expression in U251 (A), A549 (B) whole cell lysates.)

Western Blot (WB) (Western blot analysis of MRPS9 expression in U251 (A), A549 (B) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of MRPS9 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of MRPS9 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-MRPS9 antibody
Rabbit polyclonal antibody to MRPS9

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
45,835 Da
NCBI Official Full Name
28S ribosomal protein S9, mitochondrial
NCBI Official Synonym Full Names
mitochondrial ribosomal protein S9
NCBI Official Symbol
MRPS9
NCBI Official Synonym Symbols
S9mt; RPMS9; MRP-S9
NCBI Protein Information
28S ribosomal protein S9, mitochondrial
UniProt Protein Name
28S ribosomal protein S9, mitochondrial
Protein Family
UniProt Gene Name
MRPS9
UniProt Synonym Gene Names
RPMS9; MRP-S9; S9mt
UniProt Entry Name
RT09_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPS9: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. [provided by RefSeq, Jul 2008]

Protein type: Ribosomal; Mitochondrial; Translation

Chromosomal Location of Human Ortholog: 2q12.1

Cellular Component: mitochondrial inner membrane; mitochondrial small ribosomal subunit; mitochondrion; nucleolus

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: DNA damage response, detection of DNA damage; maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); mitochondrial translation; organelle organization and biogenesis; translation

Research Articles on MRPS9

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Product Notes

The MRPS9 mrps9 (Catalog #AAA8242321) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MRPS9 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MRPS9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the MRPS9 mrps9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRPS9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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