Rabbit anti-Human, Mouse HTR4 Polyclonal Antibody | anti-HTR4 antibody

HTR4, NT (HTR4, 5-hydroxytryptamine receptor 4, Serotonin receptor 4) (Biotin)

Cat. Num. AAA6308861

• Gene Names: HTR4; 5-HT4; 5-HT4R
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HTR4; Polyclonal Antibody; NT (HTR4; 5-hydroxytryptamine receptor 4; Serotonin receptor 4) (Biotin); Serotonin receptor 4; anti-HTR4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 387

• Applicable Applications for anti-HTR4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HTR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HTR4.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HTR4 Antibody (N-term) western blot analysis in MDA-MB453(lane 1) cell line and mouse liver(lane 2) tissue lysates (35ug/lane).This demonstrates the HTR4 antibody detected the HTR4 protein (arrow).)

Related Product Information for anti-HTR4 antibody

This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described.

Product Categories/Family for anti-HTR4 antibody

Antibodies; Receptors

References

Hancock, D.B., et al. Nat. Genet. 42(1):45-52(2010). Maillet, M., et al. Biochem. J. 387 (PT 2), 463-471 (2005): Brattelid, T., et al. Naunyn Schmiedebergs Arch. Pharmacol. 369(6):616-628(2004). Hiroi, T., et al. Biochem. Biophys. Res. Commun. 289(2):337-344(2001). Bender, E., et al. J. Neurochem. 74(2):478-489(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 94421454
• NCBI GeneID: 3360
• NCBI Accession #: NP_001035259.1; NP_000861.1; NP_955525.1; NP_001035262.2
• NCBI GenBank Nucleotide #: NP_001035259.1
• UniProt Accession #: Q13639
• NCBI Official Full Name: 5-hydroxytryptamine receptor 4 isoform a
• NCBI Official Synonym Full Names: 5-hydroxytryptamine receptor 4
• NCBI Official Symbol: HTR4
• NCBI Official Synonym Symbols: 5-HT4; 5-HT4R
• NCBI Protein Information: 5-hydroxytryptamine receptor 4
• UniProt Protein Name: 5-hydroxytryptamine receptor 4
• Protein Family: Transducer protein
• UniProt Gene Name: HTR4
• UniProt Synonym Gene Names: 5-HT-4; 5-HT4
• UniProt Entry Name: 5HT4R_HUMAN

NCBI Description

This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described. [provided by RefSeq, May 2010]

Uniprot Description

5-HT(4): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. Belongs to the G-protein coupled receptor 1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q31-q33

Cellular Component: membrane; integral to plasma membrane; cytoplasm; plasma membrane; endosome

Molecular Function: serotonin receptor activity

Biological Process: synaptic transmission; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cyclic nucleotide second messenger; positive regulation of cell proliferation; regulation of appetite; serotonin receptor signaling pathway