Rabbit anti-Human HTR4/5-HT4 Polyclonal Antibody | anti-HTR4 antibody

Anti-HTR4/5-HT4 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103672

• Gene Names: HTR4; 5-HT4; 5-HT4R
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: HTR4/5-HT4; Polyclonal Antibody; Anti-HTR4/5-HT4 Antibody; Rabbit Polyclonal; HTR4/5-HT4 Antibody; Rabbit PAb; Antigen Affinity Purified; 5-hydroxytryptamine (serotonin) receptor 4; G protein-coupled; Anti-5-HT4 Antibody; Anti-5-HT4R Antibody; anti-HTR4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human HTR4/5-HT4
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-HTR4 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 5-10uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the center region of the Human HTR4/5-HT4
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human HTR4/5-HT4, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-HTR4 rabbit polyclonal antibody at 1:500 dilution Lane A: SW480 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:44 kDa)

Immunoprecipitation (IP)

(HTR4 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-HTR4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-HTR4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa Observed band size :43 kDa)

Related Product Information for anti-HTR4 antibody

HTR4 (5-Hydroxytryptamine Receptor 4, also known as 5-HT4R) is a Protein Coding gene. This gene is a member of the family of serotonin receptors, which are G protein-coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The encoded protein is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. 5-HTR3 and 5-HTR4 were located in cardiac mitochondria, and regulated mitochondrial activities and cellular functions. Noncoding variants in HTR4 are associated with pulmonary function in human GWASs. The HTR4 gene is located in a region previously linked to an increased risk of asthma and atopy. SNP and haplotypes of the HTR4 gene were associated with the asthma phenotype and genetic variation of HTR4 may affect susceptibility to the development of asthma.

NCBI and Uniprot Product Information

• NCBI GI #: 11321563
• NCBI GeneID: 3360
• NCBI Accession #: NP_000861.1
• NCBI GenBank Nucleotide #: NM_000870.5
• UniProt Accession #: Q13639; Q546Q1; Q684M0; Q712M9; Q96KH9; Q96KI0; Q9H199; Q9NY73; Q9UBM6; Q9UBT4; Q9UE22; Q9UE23
• Molecular Weight: 43,761 Da
• NCBI Official Full Name: 5-hydroxytryptamine receptor 4 isoform b
• NCBI Official Synonym Full Names: 5-hydroxytryptamine (serotonin) receptor 4, G protein-coupled
• NCBI Official Symbol: HTR4
• NCBI Official Synonym Symbols: 5-HT4; 5-HT4R
• NCBI Protein Information: 5-hydroxytryptamine receptor 4; cardiac 5-HT4 receptor
• UniProt Protein Name: 5-hydroxytryptamine receptor 4
• Protein Family: Transducer protein
• UniProt Gene Name: HTR4
• UniProt Synonym Gene Names: 5-HT-4; 5-HT4
• UniProt Entry Name: 5HT4R_HUMAN

NCBI Description

This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described. [provided by RefSeq, May 2010]

Uniprot Description

5-HT(4): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. Belongs to the G-protein coupled receptor 1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q31-q33

Cellular Component: membrane; integral to plasma membrane; cytoplasm; plasma membrane; endosome

Molecular Function: serotonin receptor activity

Biological Process: synaptic transmission; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cyclic nucleotide second messenger; positive regulation of cell proliferation; regulation of appetite; serotonin receptor signaling pathway