Rabbit anti-Human HTR2C Polyclonal Antibody | anti-HTR2C antibody

HTR2C Antibody (Center)

Cat. Num. AAA9214438

• Gene Names: HTR2C; HTR1C; 5-HT1C; 5-HT2C; 5HTR2C; 5-HTR2C
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: HTR2C; Polyclonal Antibody; HTR2C Antibody (Center); 5-hydroxytryptamine receptor 2C; 5-HT-2C; 5-HT2C; 5-HTR2C; 5-hydroxytryptamine receptor 1C; 5-HT-1C; 5-HT1C; Serotonin receptor 2C; HTR1C; anti-HTR2C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This HTR2C antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 274-308 amino acids from the Central region of human HTR2C.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 248

• Applicable Applications for anti-HTR2C antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:4000
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-HTR2C Antibody (Center) at 1:4000 dilutionLane 1: SH-SY5Y whole cell lysatesLane 2: MOLT-4 whole cell lysatesLane 3: A431 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 52 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-HTR2C antibody

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1- 2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca(2+) ions from intracellular stores. Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelacortin neurons and the release of CRH that then regulates the release of corticosterone. Plays a role in the regulation of appetite and eating behavior, responses to anxiogenic stimuli and stress. Plays a role in insulin sensitivity and glucose homeostasis.

References

Saltzman A.G.,et al.Biochem. Biophys. Res. Commun. 181:1469-1478(1991).
Stam N.J.,et al.Eur. J. Pharmacol. 269:339-348(1994).
Xie E.,et al.Genomics 35:551-561(1996).
Niswender C.M.,et al.Ann. N. Y. Acad. Sci. 861:38-48(1998).
Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 4504541
• NCBI GeneID: 3358
• NCBI Accession #: NP_000859.1
• NCBI GenBank Nucleotide #: NM_000868.3
• UniProt Accession #: P28335; Q5VUF8; Q9NP28; B1AMW4
• Molecular Weight: 51821
• NCBI Official Full Name: 5-hydroxytryptamine receptor 2C isoform a
• NCBI Official Synonym Full Names: 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled
• NCBI Official Symbol: HTR2C
• NCBI Official Synonym Symbols: HTR1C; 5-HT1C; 5-HT2C; 5HTR2C; 5-HTR2C
• NCBI Protein Information: 5-hydroxytryptamine receptor 2C
• UniProt Protein Name: 5-hydroxytryptamine receptor 2C
• Protein Family: 5-hydroxytryptamine receptor
• UniProt Gene Name: HTR2C
• UniProt Synonym Gene Names: HTR1C; 5-HT-2C; 5-HT2C; 5-HTR2C; 5-HT-1C; 5-HT1C
• UniProt Entry Name: 5HT2C_HUMAN

NCBI Description

This gene encodes a seven-transmembrane G-protein-coupled receptor. The encoded protein responds to signaling through the neurotransmitter serotonin. The mRNA of this gene is subject to multiple RNA editing events, where adenosine residues encoded by the genome are converted to inosines. RNA editing is predicted to alter the structure of the second intracellular loop, thereby generating alternate protein forms with decreased ability to interact with G proteins. Abnormalities in RNA editing of this gene have been detected in victims of suicide that suffer from depression. In addition, naturally-occuring variation in the promoter and 5' non-coding and coding regions of this gene may show statistically-significant association with mental illness and behavioral disorders. Alternative splicing results in multiple different transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

5-HT(2C): a G-protein coupled receptor. One of several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. Mediates its action by activating a phosphatidylinositol-calcium second messenger system.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: serotonin binding; serotonin receptor activity; 5-HT2 receptor activity; drug binding

Biological Process: response to drug; cGMP biosynthetic process; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); behavioral fear response; regulation of corticotropin-releasing hormone secretion; serotonin receptor, phospholipase C activating pathway; locomotory behavior; positive regulation of phosphatidylinositol biosynthetic process; cellular calcium ion homeostasis; synaptic transmission; regulation of neurological process; release of sequestered calcium ion into cytosol; regulation of appetite; positive regulation of fat cell differentiation; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); feeding behavior; serotonin receptor signaling pathway