Western Blot (WB)
(Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: HTR2C antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 29 kDa Observed band size: 75 kDa)
HTR2C recombinant antibody
HTR2C Antibody
IHC: 1:50-1:200
Western Blot (WB)
(Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: HTR2C antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 29 kDa Observed band size: 75 kDa)
Western Blot (WB)
(Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: HTR2C antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 29 kDa Observed band size: 75 kDa)
Immunohistochemistry (IHC)
(IHC image diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
Immunohistochemistry (IHC)
(IHC image diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
NCBI and Uniprot Product Information
NCBI Description
Serotonin (5-hydroxytryptamine, 5-HT), a neurotransmitter, elicits a wide array of physiological effects by binding to several receptor subtypes, including the 5-HT2 family of seven-transmembrane-spanning, G-protein-coupled receptors, which activate phospholipase C and D signaling pathways. This gene encodes the 2C subtype of serotonin receptor and its mRNA is subject to multiple RNA editing events, where genomically encoded adenosine residues are converted to inosines. RNA editing is predicted to alter amino acids within the second intracellular loop of the 5-HT2C receptor and generate receptor isoforms that differ in their ability to interact with G proteins and the activation of phospholipase C and D signaling cascades, thus modulating serotonergic neurotransmission in the central nervous system. Studies in humans have reported abnormalities in patterns of 5-HT2C editing in depressed suicide victims. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
Uniprot Description
5-HT(2C): a G-protein coupled receptor. One of several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. Mediates its action by activating a phosphatidylinositol-calcium second messenger system.
Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: Xq24
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: serotonin binding; serotonin receptor activity; 5-HT2 receptor activity; drug binding
Biological Process: response to drug; cGMP biosynthetic process; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); behavioral fear response; regulation of corticotropin-releasing hormone secretion; serotonin receptor, phospholipase C activating pathway; locomotory behavior; positive regulation of phosphatidylinositol biosynthetic process; cellular calcium ion homeostasis; synaptic transmission; regulation of neurological process; release of sequestered calcium ion into cytosol; regulation of appetite; positive regulation of fat cell differentiation; feeding behavior; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); serotonin receptor signaling pathway
Research Articles on HTR2C
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Product Notes
The HTR2C htr2c (Catalog #AAA7136713) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HTR2C Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HTR2C can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:5000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the HTR2C htr2c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HTR2C, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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