Western Blot (WB)
(Western Blot analysis of HSPA14 on different lysates using anti-HSPA14 antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: COS-1)
Rabbit HSPA14 Polyclonal Antibody | anti-HSPA14 antibody
HSPA14 Polyclonal Antibody
Gene Names
HSPA14; HSP70-4; HSP70L1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
HSPA14; Polyclonal Antibody; HSPA14 Polyclonal Antibody; Heat shock 70 kDa protein 14; Heat shock 70kDa protein 14; Heat shock protein HSP60; Heat shock protein hsp70 related protein; HSP60; HSP70 4; HSP70 like protein 1; HSP70-like protein 1; HSP70L1; HSP7E; HSPA 14; MGC131990; anti-HSPA14 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
HSPA14 polyclonal antibody detects endogenous levels of HSPA14 protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
509
Applicable Applications for anti-HSPA14 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
ICC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of HSPA14 on different lysates using anti-HSPA14 antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: COS-1)
Western Blot (WB)
(Western Blot analysis of HSPA14 on different lysates using anti-HSPA14 antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: COS-1)
Immunocytochemistry (ICC)
(ICC staining HSPA14 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining HSPA14 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-HSPA14 antibody
The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multi-protein complexes, the transportation of nascent poly-peptide chains across cellular membranes and the regulation of protein folding. HSPA14 (heat shock 70kDa protein 14), also known as HSP70-4 or HSP70L1, is a 509 amino acid novel HSP protein derived from human dendritic cells. Belonging to the heat shock protein 70 family, HSPA14 is thought to promote dendritic cell maturation. It is also suggested that HSPA14 stimulates secretion of the proinflammatory cytokines interleukin 12p70 (IL-12p70), IL-1beta, TNFalpha, and the chemokines IP-10, MIP-1alpha, MIP-1beta, and normal T cell expressed and secreted (RANTES).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55kDa
NCBI Official Full Name
heat shock 70 kDa protein 14
NCBI Official Synonym Full Names
heat shock protein family A (Hsp70) member 14
NCBI Official Symbol
HSPA14
NCBI Official Synonym Symbols
HSP70-4; HSP70L1
NCBI Protein Information
heat shock 70 kDa protein 14
UniProt Protein Name
Heat shock 70 kDa protein 14
Protein Family
UniProt Gene Name
HSPA14
UniProt Synonym Gene Names
HSP60; HSP70L1
UniProt Entry Name
HSP7E_HUMAN
Uniprot Description
HSPA14: Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity. Belongs to the heat shock protein 70 family.
Protein type: Heat shock protein
Chromosomal Location of Human Ortholog: 10p13
Cellular Component: membrane; ribosome; cytosol
Molecular Function: protein binding; ATP binding
Biological Process: 'de novo' cotranslational protein folding
Research Articles on HSPA14
Similar Products
Product Notes
The HSPA14 hspa14 (Catalog #AAA3012857) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSPA14 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HSPA14 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:1,000-1:2,000 ICC: 1:50-1:200 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the HSPA14 hspa14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSPA14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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