Rabbit anti-Human, mouse Hsp 60 Polyclonal Antibody | anti-HSPA14 antibody

Hsp 60 Antibody (N-term)

Cat. Num. AAA9206330

• Gene Names: HSPA14; HSP70-4; HSP70L1
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Hsp 60; Polyclonal Antibody; Hsp 60 Antibody (N-term); Heat shock 70 kDa protein 14; HSP70-like protein 1; Heat shock protein HSP60; HSPA14; HSP60; HSP70L1; anti-HSPA14 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Hsp 60 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-109 amino acids from the N-terminal region of human Hsp 60.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 80-109
• Sequence Length: 509

• Applicable Applications for anti-HSPA14 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of Hsp 60 Antibody (N-term) in mouse lung tissue lysates (35ug/lane). Hsp (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma with Hsp 60 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-HSPA14 antibody

Hsp60 is a member of a highly conserved family which includes molecular chaperones from several species such as plant Hsp60 (known as Rubisco binding protein), GroEL, the E.coli Hsp60 and 65 kDa major antigen of mycobacteria. In eukaryotes, Hsp60 is localized in the mitochondrial matrix and in plants Hsp60 is localized in the chloroplast. Mitochondria, chloroplasts and bacteria have a common ancestry (>1 billion years) and this fact together with the high degree of homology between the divegent Hsp60s would indicate that these proteins carry out a primitive but important function which is similar to all of these different species.The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of magnesium ions and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures. These similarities are supported by recent studies where the single ring human mitochondrial homolog, Hsp60 with its co chaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co chaperonin, GroES. Consistent with their functions as chaperones, Hsp60 and Hsp10 have been suggested to act as docking molecules with a passive role in the maturation of caspase processing. Data demonstrates that recombinant Hsp60 and Hsp10 have been shown to accelerate the activation of procaspase 3 by cytochrome c and dATP in an ATP dependent manner. Hsps are intracellular proteins which are thought to serve protective functions against infection and cellular stress, however several recent studies indicate that members of the Hsp60 family are linked to a number of autoimmune diseases, artherosclerosis and chlamydial disease.

Product Categories/Family for anti-HSPA14 antibody

Signal Transduction

References

Velez,D.R., et.al., Am. J. Obstet. Gynecol. 200 (2), 209 (2009)

NCBI and Uniprot Product Information

• NCBI GI #: 82880658
• NCBI GeneID: 51182
• NCBI Accession #: NP_057383.2
• NCBI GenBank Nucleotide #: NM_016299.3
• UniProt Accession #: Q0VDF9; Q9P0X2; Q9UI07; A8K8F8; B0YIY9
• Molecular Weight: 54794
• NCBI Official Full Name: heat shock 70 kDa protein 14 isoform 1
• NCBI Official Synonym Full Names: heat shock 70kDa protein 14
• NCBI Official Symbol: HSPA14
• NCBI Official Synonym Symbols: HSP70-4; HSP70L1
• NCBI Protein Information: heat shock 70 kDa protein 14
• UniProt Protein Name: Heat shock 70 kDa protein 14
• Protein Family: Heat shock 70 kDa protein
• UniProt Gene Name: HSPA14
• UniProt Synonym Gene Names: HSP60; HSP70L1
• UniProt Entry Name: HSP7E_HUMAN

Uniprot Description

HSPA14: Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity. Belongs to the heat shock protein 70 family.

Protein type: Heat shock protein

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: membrane; ribosome; cytosol

Molecular Function: protein binding; ATP binding

Biological Process: 'de novo' cotranslational protein folding