Rabbit HSP70L1 Polyclonal Antibody | anti-HSP70L1 antibody

HSP70L1 Polyclonal Antibody

Cat. Num. AAA3011952

• Gene Names: HSPA14; HSP70-4; HSP70L1
• Reactivity: Human, Mouse, Monkey
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: HSP70L1; Polyclonal Antibody; HSP70L1 Polyclonal Antibody; Heat shock 70 kDa protein 14; HSP70-like protein 1; Heat shock protein HSP60; HSPA14; HSP60; anti-HSP70L1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HSP70L1 polyclonal antibody detects endogenous levels of HSP70L1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 509

• Applicable Applications for anti-HSP70L1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant full length Human HSP70L1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using HSPA14 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cell using HSPA14 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-HSP70L1 antibody

The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multi-protein complexes, the transportation of nascent poly-peptide chains across cellular membranes and the regulation of protein folding. HSPA14 (heat shock 70kDa protein 14), also known as HSP70-4 or HSP70L1, is a 509 amino acid novel HSP protein derived from human dendritic cells. Belonging to the heat shock protein 70 family, HSPA14 is thought to promote dendritic cell maturation. It is also suggested that HSPA14 stimulates secretion of the proinflammatory cytokines interleukin 12p70 (IL-12p70), IL-1beta, TNFalpha, and the chemokines IP-10, MIP-1alpha, MIP-1beta and normal T cell expressed and secreted (RANTES).

NCBI and Uniprot Product Information

• NCBI GI #: 82880658
• NCBI GeneID: 51182
• NCBI Accession #: NP_057383.2
• NCBI GenBank Nucleotide #: NP_057383.2
• UniProt Accession #: Q0VDF9
• Molecular Weight: ~ 55kDa
• NCBI Official Full Name: heat shock 70 kDa protein 14
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 14
• NCBI Official Symbol: HSPA14
• NCBI Official Synonym Symbols: HSP70-4; HSP70L1
• NCBI Protein Information: heat shock 70 kDa protein 14
• UniProt Protein Name: Heat shock 70 kDa protein 14
• UniProt Gene Name: HSPA14
• UniProt Synonym Gene Names: HSP60; HSP70L1
• UniProt Entry Name: HSP7E_HUMAN

Uniprot Description

HSPA14: Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity. Belongs to the heat shock protein 70 family.

Protein type: Heat shock protein

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: membrane; ribosome; cytosol

Molecular Function: protein binding; ATP binding

Biological Process: 'de novo' cotranslational protein folding