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Western Blot (WB) (Western blot analysis of HSPA14 expression in K562 (A), HeLa (B), NIH3T3 (C), rat kidney (D) whole cell lysates.)

Rabbit HSPA14 Polyclonal Antibody | anti-HSPA14 antibody

Anti-HSPA14 Antibody

Gene Names
HSPA14; HSP70-4; HSP70L1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
HSPA14; Polyclonal Antibody; Anti-HSPA14 Antibody; HSP60; HSP70L1; Heat shock 70 kDa protein 14; HSP70-like protein 1; Heat shock protein HSP60; anti-HSPA14 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of HSPA14 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
509
Applicable Applications for anti-HSPA14 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human HSPA14. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of HSPA14 expression in K562 (A), HeLa (B), NIH3T3 (C), rat kidney (D) whole cell lysates.)

Western Blot (WB) (Western blot analysis of HSPA14 expression in K562 (A), HeLa (B), NIH3T3 (C), rat kidney (D) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of HSPA14 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of HSPA14 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-HSPA14 antibody
Rabbit polyclonal antibody to HSPA14

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54,794 Da
NCBI Official Full Name
heat shock 70 kDa protein 14 isoform 1
NCBI Official Synonym Full Names
heat shock 70kDa protein 14
NCBI Official Symbol
HSPA14
NCBI Official Synonym Symbols
HSP70-4; HSP70L1
NCBI Protein Information
heat shock 70 kDa protein 14
UniProt Protein Name
Heat shock 70 kDa protein 14
Protein Family
UniProt Gene Name
HSPA14
UniProt Synonym Gene Names
HSP60; HSP70L1
UniProt Entry Name
HSP7E_HUMAN

Uniprot Description

HSPA14: Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity. Belongs to the heat shock protein 70 family.

Protein type: Heat shock protein

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: membrane; ribosome; cytosol

Molecular Function: protein binding; ATP binding

Biological Process: 'de novo' cotranslational protein folding

Research Articles on HSPA14

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Product Notes

The HSPA14 hspa14 (Catalog #AAA8204240) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HSPA14 Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's HSPA14 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000), IHC (1/100 - 1/200). Researchers should empirically determine the suitability of the HSPA14 hspa14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSPA14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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