Human Desmoglein-2 ELISA Kit | DSG2 elisa kit

Human Desmoglein-2 ELISA Kit

Cat. Num. AAA2886739

• Gene Names: DSG2; HDGC; CDHF5
• Reactivity: Human
• Synonyms: Desmoglein-2; Human Desmoglein-2 ELISA Kit; Cadherin family member 5; HDGC; DSG2; CDHF5; DSG2 elisa kit

• Reactivity: Human
• Sequence Length: 1118

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DSG2 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 116534898
• NCBI GeneID: 1829
• NCBI Accession #: NP_001934.2
• NCBI GenBank Nucleotide #: NM_001943.4
• UniProt Accession #: Q14126; Q4KKU6
• Molecular Weight: 122,294 Da
• NCBI Official Full Name: desmoglein-2 preproprotein
• NCBI Official Synonym Full Names: desmoglein 2
• NCBI Official Symbol: DSG2
• NCBI Official Synonym Symbols: HDGC; CDHF5
• NCBI Protein Information: desmoglein-2
• UniProt Protein Name: Desmoglein-2
• Protein Family: Desmoglein
• UniProt Gene Name: DSG2
• UniProt Synonym Gene Names: CDHF5
• UniProt Entry Name: DSG2_HUMAN

NCBI Description

This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. Mutations in this gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10. [provided by RefSeq, Jan 2016]

Uniprot Description

DSG2: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10); also known as arrhythmogenic right ventricular cardiomyopathy 10 (ARVC10). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. Genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB). A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

Protein type: Calcium-binding; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 18q12.1

Cellular Component: apical plasma membrane; cell surface; desmosome; integral to membrane; intercellular junction; lateral plasma membrane; plasma membrane

Molecular Function: calcium ion binding; cell adhesion molecule binding

Biological Process: apoptosis; cell adhesion; cell structure disassembly during apoptosis; homophilic cell adhesion; maternal process involved in pregnancy; programmed cell death; response to progesterone stimulus

Disease: Arrhythmogenic Right Ventricular Dysplasia, Familial, 10; Cardiomyopathy, Dilated, 1bb