Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat tissue sections (brain) staining NGF beta in cytoplasm DAB chromogenic reaction.)
Rabbit HSP40 Polyclonal Antibody
HSP40 (DnaJ Homolog Subfamily B Member 1, Heat Shock 40kD Protein 1, Heat Shock Protein 40, DnaJ Protein Homolog 1, Human DnaJ Protein 1, hDj-1, DNAJB1, DNAJ1, HDJ1, HSPF1)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
HSP40; Polyclonal Antibody; HSP40 (DnaJ Homolog Subfamily B Member 1; Heat Shock 40kD Protein 1; Heat Shock Protein 40; DnaJ Protein Homolog 1; Human DnaJ Protein 1; hDj-1; DNAJB1; DNAJ1; HDJ1; HSPF1); Anti -HSP40 (DnaJ Homolog Subfamily B Member 1; anti-HSP40 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes human HSP40. Species Crossreactivity: mouse, rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.
Applicable Applications for anti-HSP40 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunoprecipitation, and Immunohistochemistry.
Dilution: Western Blot: 1-2ug/ml
Immunohistochemistry (paraffin): 0.5-1ug/ml
Dilution: Western Blot: 1-2ug/ml
Immunohistochemistry (paraffin): 0.5-1ug/ml
Immunogen
Synthetic peptide mapping at the C-terminal end of human HSP40, different from the related rat and mouse sequence by 2aa.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with 200ul sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat tissue sections (brain) staining NGF beta in cytoplasm DAB chromogenic reaction.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat tissue sections (brain) staining NGF beta in cytoplasm DAB chromogenic reaction.)
Western Blot (WB)
(Western blot analysis of rat brain tissue lysis using NGF beta antibody.)
Western Blot (WB)
(Western blot analysis of rat brain tissue lysis using NGF beta antibody.)
Related Product Information for anti-HSP40 antibody
The Hsp40 (heat shock protein with molecular size of approximately 40kD) is one of the mammalian homologues of bacterial DnaJ heat shock protein. Ohtsuka (1993) isolated a cDNA encoding a 40kD heat-shock protein designated HSPF1. The deduced 340aa HSPF1 protein is 34% identical to E. coli DnaJ and 34% and 36% identical to HSJ1 and HSJ2, respectively. HSPF1 gene spans over 7kb and contains 3 exons and 2 introns. HSPF1 gene is mapped to chromosome 19p13.2.
Product Categories/Family for anti-HSP40 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Hsp40
Similar Products
Product Notes
The HSP40 (Catalog #AAA627428) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSP40 (DnaJ Homolog Subfamily B Member 1, Heat Shock 40kD Protein 1, Heat Shock Protein 40, DnaJ Protein Homolog 1, Human DnaJ Protein 1, hDj-1, DNAJB1, DNAJ1, HDJ1, HSPF1) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HSP40 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunoprecipitation, and Immunohistochemistry. Dilution: Western Blot: 1-2ug/ml Immunohistochemistry (paraffin): 0.5-1ug/ml. Researchers should empirically determine the suitability of the HSP40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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