Western Blot (WB)
(Western blot analysis of Parasite lysates showing detection of HSP40 protein using Rabbit Anti-HSP40 Polyclonal Antibody . Primary Antibody: Rabbit Anti-HSP40 Polyclonal Antibody at 1:2000.)
Rabbit HSP40 Polyclonal Antibody | anti-HSP40 antibody
HSP40 Antibody
Reactivity
Plasmodium Falciparum
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Protein A Purified
Synonyms
HSP40; Polyclonal Antibody; HSP40 Antibody; Rabbit Anti-P. Falciparum HSP40 Polyclonal; DNAJ1 Antibody; NDAJB1 Antibody; HDJ1 Antibody; HSPF1 Antibody; DnaJ homolog subfamily B member 1 Antibody; Dna J protein homolog 1 Antibody; Heat shock 40 kDa protein 1 Antibody; heat shock protein 40 Antibody; Human DnaJ protein 1 Antibody; hDj-1 Antibody; anti-HSP40 antibody
Host
Rabbit
Reactivity
Plasmodium Falciparum
Clonality
Polyclonal
Specificity
Detects ~ 62kDa. Specific to P. Falciparum and does not cross-react to any protein from Human erythrocytes.
Purity/Purification
Protein A Purified
Form/Format
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration
1.8 mg/ml (varies by lot)
Applicable Applications for anti-HSP40 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:2000
ICC/IF: 1:50
ICC/IF: 1:50
Immunogen
C-terminal peptide of Pf11_0513 conjugated to KLH
Immunogen Species
P. Falciparum
Conjugate
Unconjugated
Cellular Localization
Cytoplasm, Nucleus
Certificate of Analysis
0.9 ug/ml of SPC-184 was sufficient for detection of Pf11_0513 in 20 ug of P. falciparum lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Preparation and Storage
Store at-20 degree C.
Western Blot (WB)
(Western blot analysis of Parasite lysates showing detection of HSP40 protein using Rabbit Anti-HSP40 Polyclonal Antibody . Primary Antibody: Rabbit Anti-HSP40 Polyclonal Antibody at 1:2000.)
Western Blot (WB)
(Western blot analysis of Parasite lysates showing detection of HSP40 protein using Rabbit Anti-HSP40 Polyclonal Antibody . Primary Antibody: Rabbit Anti-HSP40 Polyclonal Antibody at 1:2000.)
Related Product Information for anti-HSP40 antibody
DnaJ/HSP40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, HSP70s. Because the ATP hydrolysis is essential for the activity of HSP70s, DnaJ/HSP40 proteins actually determine the activity of HSP70s by stabilizing their interaction with substrate proteins. DnaJ/HSP40 proteins all contain the J domain through which they bind to HSP70s. HSP40, also known as HDJ1 (1), is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-6). HSP 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to HSC70/HSP70, the mammalian homologues of the bacterial heat shock protein, DnaK (3). PF11_0513 belongs to the HSP40 family of chaperones. This protein has a Plasmodium export element (PEXEL). It is exported out of the parasite into the infected erythrocytic compartment.
Product Categories/Family for anti-HSP40 antibody
References
1. Ohtsuka K. (1993) Biochem. Biophys. Res. Commun. 197: 235-240. 2. Melville M.W., et al. (1997) PNAS USA. 94: 97-102. 3. Hattori H., Let al. (1992) Cell Structure and Function. 17: 77-86. 4. Ohtsuka K. Masuda A., Nakai A., and Nagata K. (1990) Biochem. Biophys. Res. Commun. 166: 642-647. 5. Bardwell J.C.A., et al. (1986) J. Biol. Chem. 261: 1782-1785. 6. Ohku M., Tamura F., Nishimura S., and Uchida H. (1986) J. Biol. Chem. 261: 1778-1781.
NCBI and Uniprot Product Information
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Product Notes
The HSP40 (Catalog #AAA8002022) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSP40 Antibody reacts with Plasmodium Falciparum and may cross-react with other species as described in the data sheet. AAA Biotech's HSP40 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:2000 ICC/IF: 1:50. Researchers should empirically determine the suitability of the HSP40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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