Western Blot (WB)
(Western blot analysis of HSP27 (pS15) expression in HeLa Anisomycin-treated (A); MCF7 (B) whole cell lysates.)
Rabbit HSP27 Polyclonal Antibody | anti-HSP27 antibody
HSP27 Antibody
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Immunogen Affinity Chromatography
Synonyms
HSP27; Polyclonal Antibody; HSP27 Antibody; Purified Polyclonal HSP27 Antibody; Polyclonal HSP27 antibody; Anti-HSP27 antibody; HSP27 antibody; HSP28 antibody; Heat shock protein beta-1 antibody; HspB1 antibody; 28 kDa heat shock protein antibody; Estrogen-regulated 24 kDa protein antibody; Heat shock 27 kDa protein antibody; HSP 27 antibody; Stress-responsive protein 27 antibody; SRP27 antibody; anti-HSP27 antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of HSP27 protein
Purity/Purification
Immunogen Affinity Chromatography
Form/Format
Supplied in liquid form in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3 with 30% Glycerol and 0.01% Sodium Azide
Applicable Applications for anti-HSP27 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IHC: 1:100-1:200
IP: 1:10-1:100
IHC: 1:100-1:200
IP: 1:10-1:100
Immunogen
HSP27 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human HSP27 as the immunogen
Cross-Reactivity
Bovine, Monkey
Preparation and Storage
Store at 4 degree C for short term storage. For long term, aliquot and store at -20 degree C. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of HSP27 (pS15) expression in HeLa Anisomycin-treated (A); MCF7 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of HSP27 (pS15) expression in HeLa Anisomycin-treated (A); MCF7 (B) whole cell lysates.)
Related Product Information for anti-HSP27 antibody
HSP27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, a-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction.
Product Categories/Family for anti-HSP27 antibody
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Product Notes
The HSP27 (Catalog #AAA5314496) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HSP27 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:500-1:1000 IHC: 1:100-1:200 IP: 1:10-1:100. Researchers should empirically determine the suitability of the HSP27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP27, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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