Western Blot (WB)
(The anti-UBE4B Pab is used in Western blot to detect UBE4B in mouse kidney tissue lysate.)
Rabbit anti-Human, Mouse UBE4B Polyclonal Antibody | anti-UBE4B antibody
UBE4B, NT (Ubiquitin Conjugation Factor E4 B, Ubiquitin-fusion Degradation Protein 2, Homozygously Deleted In Neuroblastoma-1, HDNB1, KIAA0684, UFD2) (Biotin)
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Western Blot (WB)
(The anti-UBE4B Pab is used in Western blot to detect UBE4B in mouse kidney tissue lysate.)
Western Blot (WB)
(The anti-UBE4B Pab is used in Western blot to detect UBE4B in mouse kidney tissue lysate.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
NCBI and Uniprot Product Information
NCBI Description
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
UBE4B: Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3. Belongs to the ubiquitin conjugation factor E4 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Ligase; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 1p36.3
Cellular Component: cytoplasm; nucleus; ubiquitin ligase complex
Molecular Function: enzyme binding; ligase activity
Biological Process: proteasomal ubiquitin-dependent protein catabolic process; induction of apoptosis by granzyme; protein ubiquitination during ubiquitin-dependent protein catabolic process; response to UV
Research Articles on UBE4B
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Product Notes
The UBE4B ube4b (Catalog #AAA6504674) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBE4B, NT (Ubiquitin Conjugation Factor E4 B, Ubiquitin-fusion Degradation Protein 2, Homozygously Deleted In Neuroblastoma-1, HDNB1, KIAA0684, UFD2) (Biotin) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's UBE4B can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-1:100 ELISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the UBE4B ube4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE4B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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