Rabbit anti-Human USP14 Polyclonal Antibody | anti-USP14 antibody

USP14, NT (Ubiquitin Carboxyl-terminal Hydrolase 14, Ubiquitin Thiolesterase 14, Ubiquitin-specific Processing Protease 14, Deubiquitinating Enzyme 14, TGT) (PE)

Cat. Num. AAA6505166

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: USP14; Polyclonal Antibody; NT (Ubiquitin Carboxyl-terminal Hydrolase 14; Ubiquitin Thiolesterase 14; Ubiquitin-specific Processing Protease 14; Deubiquitinating Enzyme 14; TGT) (PE); anti-USP14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human USP14.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-USP14 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human USP14 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(The anti-USP14 Pab is used in Western blot to detect USP14 in USP14-transfected HeLa cell lysates. Transfection data is kindly provided by Dr. B. Pierrat from the Novartis Institute for Biomedical Research (Basel, Switzerland).)

Western Blot (WB)

(Western blot analysis of anti-USP14 Pab in A2058 cell line lysates (35ug/lane). USP14 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot analysis of USP14 (arrow) using rabbit polyclonal USP14 Antibody (K7). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the USP14 gene.)

Product Categories/Family for anti-USP14 antibody

Antibodies; Ubiquitin

References

1. Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003). 2. D'Andrea, A., et al., Crit. Rev. Biochem. Mol. Biol. 33(5):337-352 (1998). 3. Deshpande, K.L., et al., Arch. Biochem. Biophys. 326(1):1-7 (1996).