Rabbit HSP22 Polyclonal Antibody | anti-HSP22 antibody

HSP22 Antibody

Cat. Num. AAA8002018

• Gene Names: HSPB8; H11; HMN2; CMT2L; DHMN2; E2IG1; HMN2A; HSP22
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Peptide Affinity Purified
• Synonyms: HSP22; Polyclonal Antibody; HSP22 Antibody; Rabbit Anti-Human HSP22 Polyclonal; Alpha crystallin C chain Antibody; CMT2L Antibody; CRYAC Antibody; DHMN2 Antibody; H11 Antibody; Heat shock 22kDa protein 8 Antibody; HMN2 Antibody; HSB8 Antibody; HSPB8 Antibody; anti-HSP22 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Detects ~22kDa. Does not cross-react with HSP27 or alpha-crystallin.
• Purity/Purification: Peptide Affinity Purified
• Form/Format: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 196

• Applicable Applications for anti-HSP22 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000; ICC/IF: 1:100; IHC: 1:100
• Immunogen: Human HSP22
• Immunogen Species: Human
• Conjugate: Unconjugated
• Cellular Localization: Cytoplasm, Nucleus
• Certificate of Analysis: A 1:1000 dilution was sufficient for detection of HSP22 in 10 ug of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Preparation and Storage: Store at-20 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSP22 Polyclonal Antibody . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at-20C for 10 minutes. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Nuclear Staining.)

Western Blot (WB)

(Western blot analysis of Rat Skeletal muscle lysates showing detection of HSP22 protein using Rabbit Anti-HSP22 Polyclonal Antibody . Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-HSP22 Polyclonal Antibody . Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Epidermis positive, dermal staining.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-HSP22 Polyclonal Antibody . Tissue: Spinal cord. Species: Mouse. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody at 1:100. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. DAPI merged with Alexa 488. Courtesy of: Joy Irobi-Devolder, University of Antwerp, Belgium.)

Related Product Information for anti-HSP22 antibody

HSP22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to HSP27. Similar to most other sHSPs, HSP22 is predominately transcribed in skeletal muscle and heart, as well as the placenta (1). HSP22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HSPB8 interacted preferentially with a triple aspartate form of HSP27 which mimics HSP27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type HSP27 (2). HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs (3). The chaperone-like activity is of great importance to the function of HSP22 in various processes including proliferation, apoptosis and macro autophagy (4). Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L) (5).

Product Categories/Family for anti-HSP22 antibody

Cancer; Heat Shock; Cell Signaling; Protein Trafficking; Chaperone Proteins; Neuroscience; Neurodegeneration

References

1. Kappe G., et al. (2001) Biochem Biophys Acta. 1520: 1-6. 2. Benndorf R., et al. (2001) J Biol Chem. 276: 26753-26761. 3. Sun X., et al. (2004) J Biol Chem. 279: 2394-2402. 4. Kim M.V., et al. (2004) Biochem Biophys Res Commun. 325: 649-652. 5. Wilhelmus M.M., et al. (2006) Acta Neuropathol. (Berl) 111: 139-149.

NCBI and Uniprot Product Information

• NCBI GI #: 13431576
• NCBI GeneID: 26353
• UniProt Accession #: Q9UJY1
• NCBI Official Full Name: Heat shock protein beta-8
• NCBI Official Synonym Full Names: heat shock protein family B (small) member 8
• NCBI Official Symbol: HSPB8
• NCBI Official Synonym Symbols: H11; HMN2; CMT2L; DHMN2; E2IG1; HMN2A; HSP22
• NCBI Protein Information: heat shock protein beta-8
• UniProt Protein Name: Heat shock protein beta-8
• Protein Family: Heat shock protein
• UniProt Gene Name: HSPB8
• UniProt Synonym Gene Names: CRYAC; E2IG1; HSP22; HspB8
• UniProt Entry Name: HSPB8_HUMAN

NCBI Description

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq, Jul 2008]

Uniprot Description

HSPB8: Displays temperature-dependent chaperone activity. Monomer. Interacts with HSPB1. Interacts with DNAJB6. By 17-beta-estradiol. Predominantly expressed in skeletal muscle and heart. Belongs to the small heat shock protein (HSP20) family.

Protein type: Heat shock protein

Chromosomal Location of Human Ortholog: 12q24.23

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; intracellular; nucleus

Molecular Function: identical protein binding; protein binding; protein kinase activity

Disease: Charcot-marie-tooth Disease, Axonal, Type 2l; Neuronopathy, Distal Hereditary Motor, Type Iia