Mouse Hsp22 Monoclonal Antibody | anti-HSPB8 antibody

Hsp22 Antibody

Cat. Num. AAA802747

• Gene Names: HSPB8; H11; HMN2; CMT2L; DHMN2; E2IG1; HMN2A; HSP22
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA, Immunofluorescence
• Synonyms: Hsp22; Monoclonal Antibody; Hsp22 Antibody; Alpha crystallin C chain; CMT2L; CRYAC; DHMN2; H11; Heat shock 22kDa protein 8; HMN2; HSB8; HspB8; anti-HSPB8 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone Number: 3C12-H11
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 196

• Applicable Applications for anti-HSPB8 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA), Immunofluorescence (IF)
• Application Notes: 1:2000 (WB)
• Certificate of Analysis: 1 ug/mL was sufficient for detection of Hsp22 in 20 ug of whole rat tissue extract by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-Hsp22 Monoclonal Antibody, Clone 3C12-H11. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp22 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Granular layer of the epidermis. Some dermal staining.)

Western Blot (WB)

(Western Blot analysis of Rat Cell lysates showing detection of Hsp22 protein using Mouse Anti-Hsp22 Monoclonal Antibody, Clone 3C12-H11. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp22 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Related Product Information for anti-HSPB8 antibody

Background Info: Detects endogenous and exogenous hsp22 in monomeric, dimeric and tetrameric forms in WB. Does not cross react with alpha crystallin. Exogenous hsp22 detected in dimeric form.

Scientific Background: Hsp22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to Hsp27. Similar to most other sHSPs, Hsp22 is predominately transcribed in skeletal muscle and heart, as well as the placenta (1). Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HspB8 interacted preferentially with a triple aspartate form of Hsp27 which mimics Hsp27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type Hsp27 (2). HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs (3). The chaperone-like activity is of great importance to the function of Hsp22 in various processes including proliferation, apoptosis and macro autophagy (4). Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L) (5).

Product Categories/Family for anti-HSPB8 antibody

Chaperones; Heat Shock; Trafficking

References

1.Kappe G., et al. (2001) Biochem Biophys Acta 1520: 1-6. 2.Benndorf R., et al. (2001) J Biol Chem 276: 26753- 26761. 3.Sun X., et al. (2004) J Biol Chem 279: 2394-2402. 4.Kim M.V., et al. (2004) Biochem Biophys Res Commun 325: 649-652. 5. Wilhelmus M.M., et al. (2006)Acta Neuropathol (Berl) 111: 139-149.

NCBI and Uniprot Product Information

• NCBI GI #: 7657146
• NCBI GeneID: 26353
• NCBI Accession #: NP_055180.1
• NCBI GenBank Nucleotide #: NM_014365.2
• UniProt Accession #: Q9UJY1; Q6FIH3; Q9UKS3; B2R6A6
• Molecular Weight: 21,604 Da
• NCBI Official Full Name: heat shock protein beta-8
• NCBI Official Synonym Full Names: heat shock 22kDa protein 8
• NCBI Official Symbol: HSPB8
• NCBI Official Synonym Symbols: H11; HMN2; CMT2L; DHMN2; E2IG1; HMN2A; HSP22
• NCBI Protein Information: heat shock protein beta-8; protein kinase H11; alpha-crystallin C chain; E2-induced gene 1 protein; heat shock 27kDa protein 8; small stress protein-like protein HSP22
• UniProt Protein Name: Heat shock protein beta-8
• Protein Family: Heat shock protein
• UniProt Gene Name: HSPB8
• UniProt Synonym Gene Names: CRYAC; E2IG1; HSP22; PP1629; HspB8
• UniProt Entry Name: HSPB8_HUMAN

NCBI Description

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq, Jul 2008]

Uniprot Description

HSPB8: Displays temperature-dependent chaperone activity. Monomer. Interacts with HSPB1. Interacts with DNAJB6. By 17-beta-estradiol. Predominantly expressed in skeletal muscle and heart. Belongs to the small heat shock protein (HSP20) family.

Protein type: Heat shock protein

Chromosomal Location of Human Ortholog: 12q24.23

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; intracellular; nucleus

Molecular Function: identical protein binding; protein binding; protein kinase activity

Disease: Charcot-marie-tooth Disease, Axonal, Type 2l; Neuronopathy, Distal Hereditary Motor, Type Iia