Mouse Hsp22 Monoclonal Antibody | anti-GNL3 antibody

Hsp22 Antibody: ATTO 488

Cat. Num. AAA802505

• Gene Names: GNL3; NS; E2IG3; NNP47; C77032
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA, Immunofluorescence
• Synonyms: Hsp22; Monoclonal Antibody; Hsp22 Antibody: ATTO 488; Alpha crystallin C chain; CMT2L; CRYAC; DHMN2; H11; Heat shock 22kDa protein 8; HMN2; HSB8; HspB8; anti-GNL3 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone Number: 3C12-H11
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 456

• Applicable Applications for anti-GNL3 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA), Immunofluorescence (IF)
• Application Notes: 1:2000 (WB)
• Conjugate: ATTO 488
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-Hsp22 Monoclonal Antibody, Clone 3C12-H11. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp22 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Granular layer of the epidermis. Some dermal staining.)

Western Blot (WB)

(Western Blot analysis of Rat Cell lysates showing detection of Hsp22 protein using Mouse Anti-Hsp22 Monoclonal Antibody, Clone 3C12-H11. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp22 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Related Product Information for anti-GNL3 antibody

Background Info: Detects endogenous and exogenous hsp22 in monomeric, dimeric and tetrameric forms in WB. Does not cross react with alpha crystallin. Exogenous hsp22 detected in dimeric form.

Scientific Background: Hsp22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to Hsp27. Similar to most other sHSPs, Hsp22 is predominately transcribed in skeletal muscle and heart, as well as the placenta (1). Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HspB8 interacted preferentially with a triple aspartate form of Hsp27 which mimics Hsp27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type Hsp27 (2). HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs (3). The chaperone-like activity is of great importance to the function of Hsp22 in various processes including proliferation, apoptosis and macro autophagy (4). Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L) (5).

Product Categories/Family for anti-GNL3 antibody

Heat Shock; Organelle Markers; Trafficking

References

1.Kappe G., et al. (2001) Biochem Biophys Acta 1520: 1-6. 2.Benndorf R., et al. (2001) J Biol Chem 276: 26753- 26761. 3.Sun X., et al. (2004) J Biol Chem 279: 2394-2402. 4.Kim M.V., et al. (2004) Biochem Biophys Res Commun 325: 649-652. 5. Wilhelmus M.M., et al. (2006)Acta Neuropathol (Berl) 111: 139-149.

NCBI and Uniprot Product Information

• NCBI GeneID: 26354
• UniProt Accession #: Q9UJY2; Q5T9W9; B3KQY5; Q9BVP2
• Molecular Weight: 50,219 Da
• NCBI Official Synonym Full Names: guanine nucleotide binding protein-like 3 (nucleolar)
• NCBI Official Symbol: GNL3
• NCBI Official Synonym Symbols: NS; E2IG3; NNP47; C77032
• NCBI Protein Information: guanine nucleotide-binding protein-like 3
• UniProt Protein Name: Equilibrative nucleoside transporter 1
• Protein Family: Guanine nucleotide-binding protein
• UniProt Gene Name: SLC29A1
• UniProt Synonym Gene Names: ENT1; Equilibrative NBMPR-sensitive nucleoside transporter
• UniProt Entry Name: S29A1_HUMAN

NCBI Description

The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

SLC29A1: Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs). Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.

Protein type: Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: membrane; basolateral plasma membrane; integral to plasma membrane; apical plasma membrane; plasma membrane

Molecular Function: nucleoside transmembrane transporter activity

Biological Process: lactation; nucleoside transport; uridine transport; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; sleep; regulation of excitatory postsynaptic membrane potential; transmembrane transport