Rabbit Hsp22 Polyclonal Antibody | anti-GNL3 antibody

Hsp22 Antibody: HRP

Cat. Num. AAA806828

• Gene Names: GNL3; NS; E2IG3; NNP47; C77032
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
• Synonyms: Hsp22; Polyclonal Antibody; Hsp22 Antibody: HRP; Alpha crystallin C chain; CMT2L; CRYAC; DHMN2; H11; Heat shock 22kDa protein 8; HMN2; HSB8; HspB8; anti-GNL3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Form/Format: Rabbit Antiserum
• Sequence Length: 456

• Applicable Applications for anti-GNL3 antibody: Western (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: 1:1000 (WB)
• Conjugate: HRP
• Storage Buffer: Rabbit Antiserum
• Preparation and Storage: -20 degree C

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSP22 Polyclonal Antibody (SPC-181). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody (SPC-181) at 1:100 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Nuclear Staining.)

Western Blot (WB)

(Western blot analysis of Rat Skeletal muscle lysates showing detection of HSP22 protein using Rabbit Anti-HSP22 Polyclonal Antibody (SPC-181). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody (SPC-181) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-HSP22 Polyclonal Antibody (SPC-181). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody (SPC-181) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Epidermis positive, dermal staining.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-HSP22 Polyclonal Antibody (SPC-181). Tissue: Spinal cord. Species: Mouse. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody (SPC-181) at 1:100. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. DAPI merged with Alexa 488. Courtesy of: Joy Irobi-Devolder, University of Antwerp, Belgium.)

Related Product Information for anti-GNL3 antibody

Background Info: Detects ~22kDa. Does not cross-react with Hsp27 or alpha-crystallin.

Scientific Background: Hsp22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to Hsp27. Similar to most other sHSPs, Hsp22 is predominately transcribed in skeletal muscle and heart, as well as the placenta (1). Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HspB8 interacted preferentially with a triple aspartate form of Hsp27 which mimics Hsp27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type Hsp27 (2). HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs (3). The chaperone-like activity is of great importance to the function of Hsp22 in various processes including proliferation, apoptosis and macro autophagy (4). Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L) (5).

Product Categories/Family for anti-GNL3 antibody

Chaperones; Heat Shock; Trafficking

References

1.Kappe G., et al. (2001) Biochem Biophys Acta 1520: 1- 6. 2.Benndorf R., et al. (2001) J Biol Chem 276: 26753- 26761. 3.Sun X., et al. (2004) J Biol Chem 279: 2394-2402. 4.Kim M.V., et al. (2004) Biochem Biophys Res Commun 325: 649-652. 5. Wilhelmus M.M., et al. (2006)Acta Neuropathol (Berl) 111: 139-149.

NCBI and Uniprot Product Information

• NCBI GeneID: 26354
• UniProt Accession #: Q9UJY2; Q5T9W9; B3KQY5; Q9BVP2
• Molecular Weight: 50,219 Da
• NCBI Official Synonym Full Names: guanine nucleotide binding protein-like 3 (nucleolar)
• NCBI Official Symbol: GNL3
• NCBI Official Synonym Symbols: NS; E2IG3; NNP47; C77032
• NCBI Protein Information: guanine nucleotide-binding protein-like 3
• UniProt Protein Name: Equilibrative nucleoside transporter 1
• Protein Family: Guanine nucleotide-binding protein
• UniProt Gene Name: SLC29A1
• UniProt Synonym Gene Names: ENT1; Equilibrative NBMPR-sensitive nucleoside transporter
• UniProt Entry Name: S29A1_HUMAN

NCBI Description

The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

SLC29A1: Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs). Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.

Protein type: Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: membrane; integral to plasma membrane; basolateral plasma membrane; apical plasma membrane; plasma membrane

Molecular Function: nucleoside transmembrane transporter activity

Biological Process: lactation; nucleoside transport; uridine transport; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; regulation of excitatory postsynaptic membrane potential; sleep; transmembrane transport