Western Blot (WB)
(HP1 alpha pAb tested by Western blot. Detection of HP1 alpha by Western blot analysis. Nuclear extract of HeLa cells (20 ug/lane) stained with HP1 alpha pAb at a 1:1,000 dilution.)
Rabbit anti-Human, Mouse HP1a Polyclonal Antibody | anti-HP1a antibody
HP1a antibody (pAb)
Reactivity
Human, Mouse
Applications
Western Blot
Synonyms
HP1a; Polyclonal Antibody; HP1a antibody (pAb); CBX5; HP1; Hp1a; anti-HP1a antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Form/Format
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-HP1a antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:1,000 dilution
WB: 1:500-1:1,000 dilution
Immunogen
This HP1 alpha antibody was raised against a recombinant protein corresponding to full-length recombinant mouse HP1 alpha.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(HP1 alpha pAb tested by Western blot. Detection of HP1 alpha by Western blot analysis. Nuclear extract of HeLa cells (20 ug/lane) stained with HP1 alpha pAb at a 1:1,000 dilution.)
Western Blot (WB)
(HP1 alpha pAb tested by Western blot. Detection of HP1 alpha by Western blot analysis. Nuclear extract of HeLa cells (20 ug/lane) stained with HP1 alpha pAb at a 1:1,000 dilution.)
Related Product Information for anti-HP1a antibody
Short Description: Our HP1a antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human and Mouse samples.
Background: HP1 (Heterochromatin protein 1) is a chromosomal protein that participates in both chromatin packaging and gene silencing. Three HP1 isoforms (a, b and g) occur in mammals. The chromodomain (Chromatin Organization Modifier) of HP1a is responsible for binding to Histone H3 methyl Lys9, although additional factors are required to specify the HP1 targeting. The chromoshadow domain mediates interactions with a number of nuclear proteins, including SuvH39 (a Histone H3 Lysine methyltransferase), the DNA methyltransferases DNMT1 and DNMT3A or the large subunit of the chromatin assembly factor-1 (CAF-1). Dysfunction of HP1a plays a critical role in tumorigenesis, and is implicated in the invasive/metastatic phenotype of breast cancer.
Background: HP1 (Heterochromatin protein 1) is a chromosomal protein that participates in both chromatin packaging and gene silencing. Three HP1 isoforms (a, b and g) occur in mammals. The chromodomain (Chromatin Organization Modifier) of HP1a is responsible for binding to Histone H3 methyl Lys9, although additional factors are required to specify the HP1 targeting. The chromoshadow domain mediates interactions with a number of nuclear proteins, including SuvH39 (a Histone H3 Lysine methyltransferase), the DNA methyltransferases DNMT1 and DNMT3A or the large subunit of the chromatin assembly factor-1 (CAF-1). Dysfunction of HP1a plays a critical role in tumorigenesis, and is implicated in the invasive/metastatic phenotype of breast cancer.
Product Categories/Family for anti-HP1a antibody
NCBI and Uniprot Product Information
Molecular Weight
24kDa
Protein Family
Similar Products
Product Notes
The HP1a (Catalog #AAA388446) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HP1a antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HP1a can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:1,000 dilution. Researchers should empirically determine the suitability of the HP1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HP1a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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