Human Amine oxidase [flavin-containing] A ELISA Kit | MAOA elisa kit

Human Amine oxidase [flavin-containing] A ELISA Kit

Cat. Num. AAA2884834

• Gene Names: MAOA; MAO-A
• Reactivity: Human
• Synonyms: Amine oxidase [flavin-containing] A; Human Amine oxidase [flavin-containing] A ELISA Kit; Monoamine oxidase type A; MAO-A; MAOA; 1.4.3.4; MAOA elisa kit

• Reactivity: Human
• Sequence Length: 527

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAOA elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557735
• NCBI GeneID: 4128
• NCBI Accession #: NP_000231.1
• NCBI GenBank Nucleotide #: NM_000240.3
• UniProt Accession #: P21397; Q16426; B4DF46
• Molecular Weight: 44,848 Da
• NCBI Official Full Name: amine oxidase
• NCBI Official Synonym Full Names: monoamine oxidase A
• NCBI Official Symbol: MAOA
• NCBI Official Synonym Symbols: MAO-A
• NCBI Protein Information: amine oxidase [flavin-containing] A
• UniProt Protein Name: Amine oxidase [flavin-containing] A
• Protein Family: Amine oxidase
• UniProt Gene Name: MAOA
• UniProt Synonym Gene Names: MAO-A
• UniProt Entry Name: AOFA_HUMAN

NCBI Description

This gene is one of two neighboring gene family members that encode mitochondrial enzymes which catalyze the oxidative deamination of amines, such as dopamine, norepinephrine, and serotonin. Mutation of this gene results in Brunner syndrome. This gene has also been associated with a variety of other psychiatric disorders, including antisocial behavior. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012]

Uniprot Description

MAOA: Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine. Defects in MAOA are the cause of Brunner syndrome (BRUNS). Brunner syndrome is a form of X-linked non- dysmorphic mild mental retardation. Male patients are affected by a syndrome of borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior. Belongs to the flavin monoamine oxidase family.

Protein type: Membrane protein, integral; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - histidine; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - arginine and proline; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Oxidoreductase; Amino Acid Metabolism - glycine, serine and threonine; EC 1.4.3.4

Chromosomal Location of Human Ortholog: Xp11.3

Cellular Component: integral to membrane; mitochondrial outer membrane; mitochondrion

Molecular Function: amine oxidase activity; FAD binding; serotonin binding

Biological Process: biogenic amine metabolic process; dopamine catabolic process; neurotransmitter biosynthetic process; neurotransmitter catabolic process; neurotransmitter secretion; phenylethylamine metabolic process; serotonin metabolic process; synaptic transmission; xenobiotic metabolic process

Disease: Brunner Syndrome