Mouse Glycogen Phosphorylase, Muscle ELISA Kit | PYGM elisa kit

Mouse Glycogen Phosphorylase, Muscle ELISA Kit

Cat. Num. AAA764541

• Reactivity: Mouse
• Synonyms: Glycogen Phosphorylase; Muscle; Mouse Glycogen Phosphorylase; Muscle ELISA Kit; PYGM/Myophosphorylase/Glycogen phosphorylase; muscle form; PYGM elisa kit

• Reactivity: Mouse
• Sequence Length: 754

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PYGM elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse PYGM antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse PYGM antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PYGM amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse PYGM can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 257900462
• NCBI GeneID: 5837
• NCBI Accession #: NP_001158188.1
• NCBI GenBank Nucleotide #: NM_001164716.1
• Molecular Weight: 87,317 Da
• NCBI Official Full Name: glycogen phosphorylase, muscle form isoform 2
• NCBI Official Synonym Full Names: phosphorylase, glycogen, muscle
• NCBI Official Symbol: PYGM
• NCBI Protein Information: glycogen phosphorylase, muscle form
• UniProt Protein Name: Glycogen phosphorylase, muscle form
• Protein Family: Glycogen phosphorylase
• UniProt Gene Name: PYGM
• UniProt Entry Name: PYGM_HUMAN

NCBI Description

This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]

Uniprot Description

PYGM: an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Dimers associate into a tetramer to form the enzymatically active phosphorylase A. Phosphorylation of Ser-14 converts phosphorylase B (unphosphorylated) to phosphorylase A.

Protein type: Transferase; Carbohydrate Metabolism - starch and sucrose; Phosphorylase; Endoplasmic reticulum; EC 2.4.1.1

Chromosomal Location of Human Ortholog: 11q12-q13.2

Cellular Component: cytoplasm; cytosol; sarcoplasmic reticulum

Molecular Function: AMP binding; carbohydrate binding; drug binding; glycogen phosphorylase activity; protein binding; pyridoxal phosphate binding

Biological Process: carbohydrate metabolic process; cellular calcium ion homeostasis; glucose metabolic process; glycogen catabolic process; glycogen metabolic process; response to cAMP; response to hypoxia

Disease: Glycogen Storage Disease V