Rabbit anti-Human, Mouse HNRNPL / hnRNP L Polyclonal Antibody | anti-HNRNPL antibody

Rabbit Polyclonal (IgG) to Human HNRNPL / hnRNP L

Cat. Num. AAA247000

• Gene Names: HNRNPL; HNRPL; hnRNP-L; P/OKcl.14
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: HNRNPL / hnRNP L; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human HNRNPL / hnRNP L; Anti-HNRNPL / hnRNP L Antibody (aa61-110) IHC-plus; HNRNPL; HnRNP L; HnRNP-L; HNRPL; P/OKcl.14; Human HNRNPL; hnRNP L; anti-HNRNPL antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: hnRNP L Antibody detects endogenous levels of total hnRNP L protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 589

• Applicable Applications for anti-HNRNPL antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:20000), IF (1:100 - 1:500), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human hnRNP L (aa61-110).
• Immunogen: HNRNPL / hnRNP L antibody was raised against synthetic peptide from human hnRNP L (aa61-110).
• Antigen Modification: aa61-110
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-HNRNPL / hnRNP L antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-HNRNPL / hnRNP L antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of HeLa cells, using hnRNP L Antibody. The picture on the right is treated with the synthesized peptide.)

Western Blot (WB)

(Western blot of extracts from HeLa/Jurkat/HUVEC cells, using hnRNP L Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-HNRNPL antibody

Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogeneous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L(HNRPL) is among the proteins that are stably associated with hnRNP complexes. HNRPL along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. HNRPL is present in the nucleoplasm as part of the HNRP complex.

NCBI and Uniprot Product Information

• NCBI GI #: 52632383
• NCBI GeneID: 3191
• NCBI Accession #: NP_001524.2
• NCBI GenBank Nucleotide #: NM_001533.2
• UniProt Accession #: P14866; Q9H3P3; A6ND69; A6NIT8
• Molecular Weight: 50,561 Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein L isoform a
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein L
• NCBI Official Symbol: HNRNPL
• NCBI Official Synonym Symbols: HNRPL; hnRNP-L; P/OKcl.14
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein L; hnRNP L
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein L
• Protein Family: Heterogeneous nuclear ribonucleoprotein
• UniProt Gene Name: HNRNPL
• UniProt Synonym Gene Names: HNRPL; hnRNP L
• UniProt Entry Name: HNRPL_HUMAN

NCBI Description

Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP L: This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: nucleoplasm; membrane; cytoplasm; ribonucleoprotein complex; nucleus

Molecular Function: protein binding; RNA binding; nucleotide binding

Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression