Rabbit anti-Human SIM2 Polyclonal Antibody | anti-SIM2 antibody

SIM2 Polyclonal Antibody

Cat. Num. AAA9135084

• Gene Names: SIM2; SIM; bHLHe15; HMC13F06; HMC29C01
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: SIM2; Polyclonal Antibody; SIM2 Polyclonal Antibody; bHLHe15; HMC13F06; HMC29C01; SIM; anti-SIM2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence: LSLEQVSTAKSQDSWRTALSTSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPELQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFLSTLPASGECQWHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYE
• Sequence Length: 667

• Applicable Applications for anti-SIM2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500 - 1:2000
• Immunogen: Recombinant protein of human SIM2
• Immunogen Species: Human
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Cellular Location: Nucleus
• Positive Samples: U-251MG, HepG2
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using SIM2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

Related Product Information for anti-SIM2 antibody

This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants.

Product Categories/Family for anti-SIM2 antibody

Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4827004
• NCBI GeneID: 6493
• NCBI Accession #: NP_005060.1
• NCBI GenBank Nucleotide #: NM_005069.5
• UniProt Accession #: Q14190; O60766; Q15470; Q15471; Q15472; Q15473; Q16532; Q2TBD8
• Molecular Weight: Calculated: 63kDa; 73kDa; Observed: 56kDa
• NCBI Official Full Name: single-minded homolog 2 long isoform
• NCBI Official Synonym Full Names: single-minded family bHLH transcription factor 2
• NCBI Official Symbol: SIM2
• NCBI Official Synonym Symbols: SIM; bHLHe15; HMC13F06; HMC29C01
• NCBI Protein Information: single-minded homolog 2
• UniProt Protein Name: Single-minded homolog 2
• Protein Family: Single-minded
• UniProt Gene Name: SIM2
• UniProt Synonym Gene Names: BHLHE15; bHLHe15

NCBI Description

This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]

Uniprot Description

Transcription factor that may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.