Mouse anti-Human HLRC1 Polyclonal Antibody | anti-DOHH antibody

HLRC1 (Deoxyhypusine Hydroxylase, hDOHH, Deoxyhypusine Dioxygenase, Deoxyhypusine Monooxygenase, HEAT-like Repeat-containing Protein 1, DOHH, MGC4293)

Cat. Num. AAA645239

• Gene Names: DOHH; HLRC1; hDOHH
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: HLRC1; Polyclonal Antibody; HLRC1 (Deoxyhypusine Hydroxylase; hDOHH; Deoxyhypusine Dioxygenase; Deoxyhypusine Monooxygenase; HEAT-like Repeat-containing Protein 1; DOHH; MGC4293); Anti -HLRC1 (Deoxyhypusine Hydroxylase; anti-DOHH antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HLRC1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MVTEQEVDAIGQTLVDPKQPLQARFRALFTLRGLGGPGAIAWISQAFDDDSALLKHELAYCLGQMQDARAIPMLVDVLQDTRQEPMVRHEAGEALGAIGDPEVLEILKQYSSDPVIEVAETCQLAVRRLEWLQQHGGEPAAGPYLSVDPAPPAEERDVGRLREALLDESRPLFERYRAMFALRNAGGEEAALALAEGLHCGSALFRHEVGYVLGQLQHEAAVPQLAAALARCTENPMVRHECAEALGAIARPACLAALQAHADDPERVVRESCEVALDMYEHETGRAFQYADGLEQLRGAPS

• Applicable Applications for anti-DOHH antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human HLRC1, aa1-302 (NP_112594).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of HLRC1 expression in transfected 293T cell line by HLRC1 polyclonal antibody. Lane 1: HLRC1 transfected lysate (33.22kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-DOHH antibody

Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.

Product Categories/Family for anti-DOHH antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 119589723
• NCBI GeneID: 83475
• UniProt Accession #: Q9BU89; O75265
• Molecular Weight: 32,904 Da
• NCBI Official Full Name: HEAT-like (PBS lyase) repeat containing 1, isoform CRA_b
• NCBI Official Synonym Full Names: deoxyhypusine hydroxylase/monooxygenase
• NCBI Official Symbol: DOHH
• NCBI Official Synonym Symbols: HLRC1; hDOHH
• NCBI Protein Information: deoxyhypusine hydroxylase; deoxyhypusine dioxygenase; deoxyhypusine monooxygenase; HEAT-like repeat-containing protein 1; HEAT-like (PBS lyase) repeat containing 1
• UniProt Protein Name: Deoxyhypusine hydroxylase
• Protein Family: Deoxyhypusine hydroxylase
• UniProt Gene Name: DOHH
• UniProt Synonym Gene Names: HLRC1; hDOHH
• UniProt Entry Name: DOHH_HUMAN

NCBI Description

This gene encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2009]

Uniprot Description

DOHH: Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)- L-lysine intermediate to form hypusine, an essential post- translational modification only found in mature eIF-5A factor. Belongs to the deoxyhypusine hydroxylase family.

Protein type: EC 1.14.99.29; Oxidoreductase

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytosol

Molecular Function: protein binding; metal ion binding; deoxyhypusine monooxygenase activity

Biological Process: cellular protein metabolic process; hypusine biosynthetic process from peptidyl-lysine; post-translational protein modification