Immunofluorescence (IF)
(Immunofluorescence staining of PC3 cells with MBS7110487 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Rabbit anti-Human DOHH Polyclonal Antibody | anti-DOHH antibody
DOHH Antibody
Gene Names
DOHH; HLRC1; hDOHH
Reactivity
Human
Applications
ELISA, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
DOHH; Polyclonal Antibody; DOHH Antibody; Deoxyhypusine hydroxylase (hDOHH) (EC 1.14.99.29) (Deoxyhypusine dioxygenase) (Deoxyhypusine monoxygenase) (HEAT-like repeat-containing protein 1); HLRC1; anti-DOHH antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
302
Applicable Applications for anti-DOHH antibody
ELISA (EIA), Immunofluorescence (IF)
Application Notes
IF: 1:50-1:200
Immunogen
Recombinant Human Deoxyhypusine hydroxylase protein (79-159AA)
Immunogen Species
Homo sapiens (Human)
Research Area
Signal Transduction
Target Name
DOHH
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
Immunofluorescence (IF)
(Immunofluorescence staining of PC3 cells with MBS7110487 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of PC3 cells with MBS7110487 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Related Product Information for anti-DOHH antibody
Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.
Product Categories/Family for anti-DOHH antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
deoxyhypusine hydroxylase
NCBI Official Synonym Full Names
deoxyhypusine hydroxylase
NCBI Official Symbol
DOHH
NCBI Official Synonym Symbols
HLRC1; hDOHH
NCBI Protein Information
deoxyhypusine hydroxylase
UniProt Protein Name
Deoxyhypusine hydroxylase
Protein Family
UniProt Gene Name
DOHH
UniProt Synonym Gene Names
hDOHH
NCBI Description
This gene encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2009]
Uniprot Description
Catalyzes the hydroxylation of the N6-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.
Research Articles on DOHH
Similar Products
Product Notes
The DOHH dohh (Catalog #AAA7110487) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DOHH Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DOHH can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF). IF: 1:50-1:200. Researchers should empirically determine the suitability of the DOHH dohh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DOHH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
