Mouse anti-Human Melanotransferrin Polyclonal Antibody | anti-MFI2 antibody

Melanotransferrin (Melanoma-associated Antigen p97, CD228, MFI2, MAP97)

Cat. Num. AAA643892

• Gene Names: MFI2; MTF1; CD228; MAP97
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Melanotransferrin; Polyclonal Antibody; Melanotransferrin (Melanoma-associated Antigen p97; CD228; MFI2; MAP97); Anti -Melanotransferrin (Melanoma-associated Antigen p97; anti-MFI2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MFI2.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MRGPSGALWLLLALRTVLGGMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSADHCVQLIAAQEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGWNVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDESPSRRQTWTRSEEEEGECPAHEEARRTMRSSAGQAWKWAPVHRPQDESDKGEFGKRAKSRDMLG

• Applicable Applications for anti-MFI2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human MFI2, aa1-302 (NP_201573.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MFI2 polyclonal antibody. Western Blot analysis of MFI2 expression in human pancreas.)

Western Blot (WB)

(Western Blot analysis of MFI2 expression in transfected 293T cell line by MFI2 polyclonal antibody. Lane 1: MFI2 transfected lysate (33.22KD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-MFI2 antibody

The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. It is involved in iron cellular uptake and seems to be internalized and then recycled back to the cell membrane. It binds a single atom of iron per subunit and could also bind zinc. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. There are two named isoforms.

Product Categories/Family for anti-MFI2 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 16163666
• NCBI GeneID: 4241
• NCBI Accession #: NP_201573.1
• NCBI GenBank Nucleotide #: NM_033316.3
• UniProt Accession #: P08582; Q9BQE2
• Molecular Weight: 80,215 Da
• NCBI Official Full Name: melanotransferrin isoform 2
• NCBI Official Synonym Full Names: antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
• NCBI Official Symbol: MFI2
• NCBI Official Synonym Symbols: MTF1; CD228; MAP97
• NCBI Protein Information: melanotransferrin; membrane-bound transferrin-like protein; melanoma-associated antigen p97, isoform 2
• UniProt Protein Name: Melanotransferrin
• Protein Family: Melanotransferrin
• UniProt Gene Name: MFI2
• UniProt Synonym Gene Names: MAP97
• UniProt Entry Name: TRFM_HUMAN

NCBI Description

The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

MFI2: Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 3q28-q29

Cellular Component: anchored to plasma membrane; cell surface; integral to plasma membrane

Molecular Function: protein binding; ferric iron binding; iron ion binding

Biological Process: cellular iron ion homeostasis; regulation of cell growth; regulation of cell proliferation