Rabbit anti-Human HLA-DOB Polyclonal Antibody | anti-HLA-DOB antibody

HLA-DOB Antibody

Cat. Num. AAA7131387

• Gene Names: HLA-DOB; DOB
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: HLA-DOB; Polyclonal Antibody; HLA-DOB Antibody; HLA class II histocompatibility antigen; DO beta chain; MHC class II antigen DOB; anti-HLA-DOB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid; Rabbit IgG In Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol.

• Applicable Applications for anti-HLA-DOB antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:500-1:3000
• Immunogen: Synthesized peptide derived from N-terminal of human HLA-DOB.
• Immunogen Species: Human
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blot analysis of extracts from 293 cell, using HLA-DOB antibody. )

Product Categories/Family for anti-HLA-DOB antibody

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 4504403
• NCBI GeneID: 3112
• NCBI Accession #: NP_002111.1
• NCBI GenBank Nucleotide #: NM_002120.3
• UniProt Accession #: P13765; Q29746; Q29825; Q6FHC2; B0V0Y0
• Molecular Weight: 30,822 Da
• NCBI Official Full Name: HLA class II histocompatibility antigen, DO beta chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DO beta
• NCBI Official Symbol: HLA-DOB
• NCBI Official Synonym Symbols: DOB
• NCBI Protein Information: HLA class II histocompatibility antigen, DO beta chain; MHC class II antigen DOB
• UniProt Protein Name: HLA class II histocompatibility antigen, DO beta chain
• Protein Family: HLA class II histocompatibility antigen
• UniProt Gene Name: HLA-DOB
• UniProt Entry Name: DOB_HUMAN

NCBI Description

HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-DOB: Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM. Belongs to the MHC class II family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: lysosomal membrane; lysosome; integral to membrane; endosome membrane; MHC class II protein complex

Molecular Function: MHC class II receptor activity

Biological Process: negative regulation of antigen processing and presentation of peptide antigen via MHC class II; antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; signal transduction