Human Chemokine C C Motif Ligand 14 ELISA Kit | CCL14 elisa kit

Human Chemokine C C Motif Ligand 14 ELISA Kit

Cat. Num. AAA727710

• Gene Names: CCL14; CC-1; CC-3; CKB1; MCIF; NCC2; SY14; HCC-1; HCC-3; NCC-2; SCYL2; SCYA14; HCC-1(1-74); HCC-1/HCC-3
• Reactivity: Human
• Synonyms: Chemokine C C Motif Ligand 14; Human Chemokine C C Motif Ligand 14 ELISA Kit; CCL14 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CCL14. No significant cross-reactivity or interference between CCL14 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CCL14 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive or Sandwich
• Sensitivity: 1.0 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL14 elisa kit

Intended Uses: This CCL14 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CCL14. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: CCL14 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CCL14 antibody and an CCL14-HRP conjugate. The assay sample and buffer are incubated together with CCL14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCL14 concentration since CCL14 from samples and CCL14-HRP conjugate compete for the anti-CCL14 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCL14 from the sample, fewer sites are left to bind CCL14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCL14 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CCL14 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 14589961
• NCBI GeneID: 6358
• NCBI Accession #: NP_116739.1
• NCBI GenBank Nucleotide #: NM_032963.3
• Molecular Weight: 12,297 Da
• NCBI Official Full Name: C-C motif chemokine 14 isoform 1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 14
• NCBI Official Symbol: CCL14
• NCBI Official Synonym Symbols: CC-1; CC-3; CKB1; MCIF; NCC2; SY14; HCC-1; HCC-3; NCC-2; SCYL2; SCYA14; HCC-1(1-74); HCC-1/HCC-3
• NCBI Protein Information: C-C motif chemokine 14; chemokine CC-3; new CC chemokine 2; chemokine CC-1/CC-3; hemofiltrate CC chemokine 1; small-inducible cytokine A14; small inducible cytokine subfamily A (Cys-Cys), member 14
• UniProt Protein Name: C-C motif chemokine 14
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL14
• UniProt Synonym Gene Names: NCC2; SCYA14; HCC-1/HCC-3
• UniProt Entry Name: CCL14_HUMAN

NCBI Description

This gene, chemokine (C-C motif) ligand 14, is one of several CC cytokine genes clustered on 17q11.2. The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Read-through transcripts are also expressed that include exons from the upstream cytokine gene, chemokine (C-C motif) ligand 15, and are represented as GeneID: 348249. [provided by RefSeq, Dec 2009]

Uniprot Description

CCL14: Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space

Molecular Function: chemokine activity

Biological Process: cellular calcium ion homeostasis; positive regulation of cell proliferation; immune response