Rabbit anti-Human HK2 Polyclonal Antibody | anti-HK2 antibody

Anti-HK2 Antibody

Cat. Num. AAA416290

• Gene Names: ATP2A2; DD; DAR; ATP2B; SERCA2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: HK2; Polyclonal Antibody; Anti-HK2 Antibody; anti-HK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 997

• Applicable Applications for anti-HK2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA titer: 1:20,000-1:80,000; Western blot analysis: 1:500-1:1,000; Immunohistochemistry: 1:100-500; Optimal dilutions/concentrations should be determined by the end user
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-HK2 antibody

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]

References

(1) Neary,C.L. and Pastorino,J.G. Nucleocytoplasmic shuttling of hexokinase II in a cancer cell. Biochem. Biophys. Res. Commun. 394 (4), 1075-1081 (2010)

NCBI and Uniprot Product Information

• NCBI GI #: 306851
• NCBI GeneID: 488
• Molecular Weight: 109,734 Da
• NCBI Official Full Name: HK2
• NCBI Official Synonym Full Names: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
• NCBI Official Symbol: ATP2A2
• NCBI Official Synonym Symbols: DD; DAR; ATP2B; SERCA2
• NCBI Protein Information: sarcoplasmic/endoplasmic reticulum calcium ATPase 2; ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2; SR Ca(2+)-ATPase 2; calcium pump 2; calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; cardiac Ca2+ ATPase; endoplasmic reticulum class 1/2 Ca(2+) ATPase
• UniProt Protein Name: Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
• Protein Family: Probable histidine kinase
• UniProt Gene Name: ATP2A2
• UniProt Entry Name: AT2A2_HUMAN

NCBI Description

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

SERCA2: an intracellular pump located in the sarcoplasmic or endoplasmic reticula of muscle cells. Possesses a Ca(2+)-ATPase activity. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN. Two alternatively spliced isoforms have been described.

Protein type: Hydrolase; Transporter; Membrane protein, integral; Cell adhesion; Transporter, ion channel; Endoplasmic reticulum; EC 3.6.3.8; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; membrane; sarcoplasmic reticulum; endoplasmic reticulum; integral to plasma membrane

Molecular Function: protein C-terminus binding; protein binding; enzyme binding; calcium-transporting ATPase activity; metal ion binding; calcium ion binding; ATP binding

Biological Process: cellular calcium ion homeostasis; ER-nuclear signaling pathway; endoplasmic reticulum calcium ion homeostasis; epidermis development; negative regulation of heart contraction; metabolic process; elevation of endoplasmic reticulum calcium ion concentration; positive regulation of heart rate; cell adhesion; blood coagulation; transmembrane transport; regulation of the force of heart contraction

Disease: Acrokeratosis Verruciformis; Darier-white Disease