Mouse anti-Human HK2 Monoclonal Antibody | anti-HK2 antibody

Anti-HK2 Mouse mAb

Cat. Num. AAA476056

• Gene Names: ATP2A2; DD; DAR; ATP2B; SERCA2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Synonyms: HK2; Monoclonal Antibody; Anti-HK2 Mouse mAb; HKII; HXK2; DKFZp686M1669; anti-HK2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3D3
• Form/Format: Ascitic fluid containing 0.03% sodium azide.
• Sequence Length: 997

• Applicable Applications for anti-HK2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Immunogen: Purified recombinant fragment of human HK2 expressed in E Coli.

Western Blot (WB)

(Western blot analysis using HK2 mouse mAb against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of K562 cells using HK2 mouse mAb (green) and negative control (purple).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues (left) and human lung cancer (right) using HK2 mouse mAb with DAB staining.)

Related Product Information for anti-HK2 antibody

Entrez Summary: The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose- 6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases.Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

NCBI and Uniprot Product Information

• NCBI GI #: 306851
• NCBI GeneID: 488
• Molecular Weight: 102
• NCBI Official Full Name: HK2
• NCBI Official Synonym Full Names: ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
• NCBI Official Symbol: ATP2A2
• NCBI Official Synonym Symbols: DD; DAR; ATP2B; SERCA2
• NCBI Protein Information: sarcoplasmic/endoplasmic reticulum calcium ATPase 2
• UniProt Protein Name: Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
• Protein Family: Probable histidine kinase
• UniProt Gene Name: ATP2A2
• UniProt Synonym Gene Names: ATP2B; SERCA2; SR Ca(2+)-ATPase 2

NCBI Description

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

SERCA2: an intracellular pump located in the sarcoplasmic or endoplasmic reticula of muscle cells. Possesses a Ca(2+)-ATPase activity. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN. Two alternatively spliced isoforms have been described.

Protein type: Cell adhesion; EC 3.6.3.8; Endoplasmic reticulum; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ion channel

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to plasma membrane; membrane; sarcoplasmic reticulum; sarcoplasmic reticulum membrane

Molecular Function: calcium ion binding; calcium-transporting ATPase activity; enzyme binding; protein binding

Biological Process: cell adhesion; elevation of endoplasmic reticulum calcium ion concentration; endoplasmic reticulum calcium ion homeostasis; epidermis development; positive regulation of heart rate

Disease: Acrokeratosis Verruciformis; Darier-white Disease